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HelpTest Code AERID Organism Referred for Identification, Aerobic Bacteria
Infectious
Reporting Name
Organism Refer for ID, Aerobic BactUseful For
Identification of pure isolates of aerobic bacteria
Differentiation of members of the Staphylococcus aureus complex (S aureus, S argenteus, S schweitzeri)
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
VariesAdvisory Information
If susceptibility testing is needed; also order ZMMLS Antimicrobial Susceptibility, Aerobic Bacteria, MIC. If susceptibilities are not appropriate and will not be performed, ZMMLS will be canceled at report time.
Shipping Instructions
1. See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.
2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance, if appropriate.
Necessary Information
1. Specimen source is required.
2. Isolate description is required including: Gram stain reaction, morphology, and tests performed.
Specimen Required
Supplies: Infectious Container, Large (T146)
Specimen Type: Pure culture of organism from source cultured
Container/Tube: Agar slant or other appropriate media
Specimen Volume: Entire specimen
Collection Instructions:
1. Perform isolation of infecting bacteria.
2. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures.
Specimen Minimum Volume
NA
Testing Algorithm
When this test is ordered, the reflex tests may be performed and charged. All aerobically growing bacteria submitted will be identified and billed as appropriate.
Note: Mayo Clinic Laboratories recommends referring suspect select agent isolates (eg, Bacillus anthracis, Brucella species, Burkholderia mallei, Burkholderia pseudomallei, Francisella tularensis, or Yersinia pestis) to your state health department or the CDC for identification confirmation or exclusion.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient (preferred) | |
| Refrigerated | ||
Special Instructions
Reference Values
Identification of organism
Day(s) and Time(s) Performed
Monday through Sunday; Continuously
Test Classification
This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
87077-Organism Referred for Identification, Aerobic Bacteria
87077-Identification Commercial Kit (if appropriate)
87077-Ident by MALDI-TOF mass spec (if appropriate)
87077-Bacteria Identification (if appropriate)
87153-Aerobe Ident by Sequencing (if appropriate)
87077-Additional Identification Procedure (if appropriate)
87147 x 3-Serologic Agglut Method 1 Ident (if appropriate)
87147-Serologic Agglut Method 2 Ident (if appropriate)
87147 x 4-Serologic Agglut Method 3 Ident (if appropriate)
87147 x 2-6 - Serologic Agglut Method 4 Ident (if appropriate)
87077-Identification Staphylococcus (if appropriate)
87077-Identification Streptococcus (if appropriate)
87798-Identification by PCR (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| IDENT | Organism Refer for ID, Aerobic Bact | 32367-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| IDENT | Organism Refer for ID, Aerobic Bact | 32367-5 |
Clinical Reference
1. Manual of Clinical Microbiology. 12th edition. Edited by KC Carroll, MA Pfaller.. Washington, DC, ASM Press, 2019
2. Koneman's Color Atlas and Textbook of Diagnostic Microbiology. Seventh edition. Edited by GW Procop, DL Church, GS Hall, et al. Philadelphia, Lippincott Williams and Wilkins. 2017
Method Description
Pure isolates of aerobic organisms received on slants are inoculated onto culture plates based on Gram-stain morphology, the source of the isolate, clinical history, and previous results submitted by the referring client. In general, routine media utilized includes chocolate blood agar, sheep blood agar, and eosin methylene blue agar. After incubation at 35° C in 5% CO(2), the organism is identified using one or a combination of the following techniques: commercial identification strips or panels, MALDI-TOF mass spectrometry, conventional biochemical tests, and nucleic acid sequencing of the 16S ribosomal RNA (rRNA) gene.(RS Weyant, CW Moss, RE Weaver, et al: Identification of unusual pathogenic gram negative aerobic and facultatively anaerobic bacteria. CDC, Atlanta, GA, 1996; Bergey's Manual of Systematic Bacteriology, Vol 1. Edited by NR Krieg. Baltimore, MD, Williams and Wilkins, 1984; Kolbert CP, Persing DH: Ribosomal DNA sequencing as a tool for identification of bacterial pathogens. Curr Opin Microbiol 1999;2:299-305)
Analytic Time
5 daysReject Due To
| Other | Agar plate |
Method Name
Dependent on organism submitted, 1 or more of the following methods will be used: conventional biochemical testing, commercial identification strips or panels, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) mass spectrometry, and 16S RNA gene sequencing.
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| COMM | Identification Commercial Kit | No, (Bill Only) | No |
| RMALD | Ident by MALDI-TOF mass spec | No, (Bill Only) | No |
| GID | Bacteria Identification | No, (Bill Only) | No |
| ISAE | Aerobe Ident by Sequencing | No, (Bill Only) | No |
| REFID | Additional Identification Procedure | No, (Bill Only) | No |
| SALS | Serologic Agglut Method 1 Ident | No, (Bill Only) | No |
| EC | Serologic Agglut Method 2 Ident | No, (Bill Only) | No |
| SHIG | Serologic Agglut Method 3 Ident | No, (Bill Only) | No |
| STAP | Identification Staphylococcus | No, (Bill Only) | No |
| STRP | Identification Streptococcus | No, (Bill Only) | No |
| SIDC | Ident Serologic Agglut Method 4 | No, (Bill Only) | No |
| PCRID | Identification by PCR | No, (Bill Only) | No |