Sign in →

Test Code AERID Organism Referred for Identification, Aerobic Bacteria


Reporting Name

Organism Refer for ID, Aerobic Bact

Useful For

Identification of pure isolates of aerobic bacteria


Differentiation of members of the Staphylococcus aureus complex (S aureus, S argenteus, S schweitzeri)

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type


Ordering Guidance

If susceptibility testing is needed; also order ZMMLS Antimicrobial Susceptibility, Aerobic Bacteria, MIC. If susceptibilities are not appropriate and will not be performed, ZMMLS will be canceled at report time.

Shipping Instructions

1. See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.

2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance, if appropriate.

Necessary Information

1. Specimen source is required.

2. Isolate description is required including: Gram stain reaction, morphology, and tests performed.

Specimen Required

Supplies: Infectious Container, Large (T146)

Specimen Type: Pure culture of organism from source cultured

Container/Tube: Agar slant or other appropriate media

Specimen Volume: Entire specimen

Collection Instructions:

1. Perform isolation of infecting bacteria.

2. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures.

Specimen Minimum Volume


Testing Algorithm

When this test is ordered, the reflex tests may be performed and charged. All aerobically growing bacteria submitted will be identified and billed as appropriate.

Note: Mayo Clinic Laboratories recommends referring suspect select agent isolates (eg, Bacillus anthracis, Brucella species, Burkholderia mallei, Burkholderia pseudomallei, Francisella tularensis, or Yersinia pestis) to your state health department or the CDC for identification confirmation or exclusion.

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)

Reference Values

Identification of organism

Day(s) Performed

Monday through Sunday

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87077-Organism Referred for Identification, Aerobic Bacteria

87077-Identification Commercial Kit (if appropriate)

87077-Ident by MALDI-TOF mass spec (if appropriate)

87077-Bacteria Identification (if appropriate)

87153-Aerobe Ident by Sequencing (if appropriate)

87077-Additional Identification Procedure (if appropriate)

87147 x 3-Serologic Agglut Method 1 Ident (if appropriate)

87147-Serologic Agglut Method 2 Ident (if appropriate)

87147 x 4-Serologic Agglut Method 3 Ident (if appropriate)

87147 x 2-6 - Serologic Agglut Method 4 Ident (if appropriate)

87077-Identification Staphylococcus (if appropriate)

87077-Identification Streptococcus (if appropriate)

87798-Identification by PCR (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
IDENT Organism Refer for ID, Aerobic Bact 32367-5


Result ID Test Result Name Result LOINC Value
IDENT Organism Refer for ID, Aerobic Bact 32367-5

Clinical Reference

1. Manual of Clinical Microbiology. 12th edition. Edited by KC Carroll, MA Pfaller.. Washington, DC, ASM Press, 2019

2. Koneman's Color Atlas and Textbook of Diagnostic Microbiology. Seventh edition. Edited by GW Procop, DL Church, GS Hall, et al. Philadelphia, Lippincott Williams and Wilkins. 2017

Method Description

Pure isolates of aerobic organisms received on slants are inoculated onto culture plates based on Gram-stain morphology, the source of the isolate, clinical history, and previous results submitted by the referring client. In general, routine media utilized includes chocolate blood agar, sheep blood agar, and eosin methylene blue agar. After incubation at 35° C in 5% CO(2), the organism is identified using one or a combination of the following techniques: commercial identification strips or panels, MALDI-TOF mass spectrometry, conventional biochemical tests, and nucleic acid sequencing of the 16S ribosomal RNA (rRNA) gene.(RS Weyant, CW Moss, RE Weaver, et al: Identification of unusual pathogenic gram negative aerobic and facultatively anaerobic bacteria. CDC, Atlanta, GA, 1996; Bergey's Manual of Systematic Bacteriology, Vol 1. Edited by NR Krieg. Baltimore, MD, Williams and Wilkins, 1984; Kolbert CP, Persing DH: Ribosomal DNA sequencing as a tool for identification of bacterial pathogens. Curr Opin Microbiol 1999;2:299-305)

Report Available

5 to 10 days

Reject Due To

Other Agar plate

Method Name

Dependent on organism submitted, 1 or more of the following methods will be used: conventional biochemical testing, commercial identification strips or panels, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) mass spectrometry, and 16S RNA gene sequencing.


If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
COMM Identification Commercial Kit No, (Bill Only) No
RMALD Ident by MALDI-TOF mass spec No, (Bill Only) No
GID Bacteria Identification No, (Bill Only) No
ISAE Aerobe Ident by Sequencing No, (Bill Only) No
REFID Additional Identification Procedure No, (Bill Only) No
SALS Serologic Agglut Method 1 Ident No, (Bill Only) No
EC Serologic Agglut Method 2 Ident No, (Bill Only) No
SHIG Serologic Agglut Method 3 Ident No, (Bill Only) No
STAP Identification Staphylococcus No, (Bill Only) No
STRP Identification Streptococcus No, (Bill Only) No
SIDC Ident Serologic Agglut Method 4 No, (Bill Only) No
PCRID Identification by PCR No, (Bill Only) No

Secondary ID