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Test Code ALDAS Aldolase, Serum

Additional Codes

Mayo: ALS

Reporting Name

Aldolase, S

Useful For

Detection of muscle disease

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum Red

Specimen Required

Collection Container/Tube: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Centrifuge within 1 hour of collection and aliquot serum into plastic vial.

2. Send refrigerated.

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Red Refrigerated (preferred) 7 days
  Frozen  60 days

Reference Values

<18 years: <14.5 U/L

≥18 years: <7.7 U/L

Day(s) Performed

Monday through Saturday

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
ALS Aldolase, S 1761-6


Result ID Test Result Name Result LOINC Value
ALS Aldolase, S 1761-6

Clinical Reference

1. Bohlmeyer TJ, Wu AH, Perryman MB: Evaluation of laboratory tests as a guide to diagnosis and therapy of myositis. Rheum Dis Clin of North Am. 1994 Nov;20(4):845-856

2. Bohan A, Peter JB, Bowman RL, Pearson CM: Computer-assisted analysis of 153 patients with polymyositis and dermatomyositis. Medicine (Baltimore). 1977 Jul;56(4):255-286. doi: 10.1097/00005792-197707000-00001

3. Thompson RA, Vignos PJ Jr: Serum aldolase in muscle disease. AMA Arch Intern Med. 1959 Apr;103(4):551-564. doi: 10.1001/archinte.1959.00270040037004

4. Ganguly A: Management of muscular dystrophy during osteoarthritis disorder: A topical phytotherapeutic treatment protocol. Caspian J Intern Med. 2019;10(2):183-196. doi: 10.22088/cjim.10.2.183

Method Description

The aldolase activity is determined from the rate at which NADH is oxidized to NAD and is measured photometrically by a decrease in absorbance. For each mole of substrate hydrolyzed, 2 moles of coenzyme (NADH) are oxidized. This 1:2 ratio is applied in the calculation of aldolase activity. Lactate dehydrogenase (LDH) is present in the reagent for the purpose of eliminating interference from endogenous pyruvate in the specimen.(Package insert: Roche Aldolase Reagent. Roche Diagnostics; V 4.0, 05/2017)

Report Available

1 to 3 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Method Name



If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.

Secondary ID