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HelpTest Code ALDAS Aldolase, Serum
Additional Codes
Mayo: ALS
Reporting Name
Aldolase, SUseful For
Detection of muscle disease
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Serum RedSpecimen Required
Collection Container/Tube: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Centrifuge within 1 hour of collection and aliquot serum into plastic vial.
2. Send refrigerated.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum Red | Refrigerated (preferred) | 7 days | |
| Frozen | 60 days |
Reference Values
<18 years: <14.5 U/L
≥18 years: <7.7 U/L
Day(s) Performed
Monday through Saturday
Test Classification
This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
82085
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ALS | Aldolase, S | 1761-6 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| ALS | Aldolase, S | 1761-6 |
Clinical Reference
1. Bohlmeyer TJ, Wu AH, Perryman MB: Evaluation of laboratory tests as a guide to diagnosis and therapy of myositis. Rheum Dis Clin of North Am. 1994 Nov;20(4):845-856
2. Bohan A, Peter JB, Bowman RL, Pearson CM: Computer-assisted analysis of 153 patients with polymyositis and dermatomyositis. Medicine (Baltimore). 1977 Jul;56(4):255-286. doi: 10.1097/00005792-197707000-00001
3. Thompson RA, Vignos PJ Jr: Serum aldolase in muscle disease. AMA Arch Intern Med. 1959 Apr;103(4):551-564. doi: 10.1001/archinte.1959.00270040037004
4. Ganguly A: Management of muscular dystrophy during osteoarthritis disorder: A topical phytotherapeutic treatment protocol. Caspian J Intern Med. 2019;10(2):183-196. doi: 10.22088/cjim.10.2.183
Method Description
The aldolase activity is determined from the rate at which NADH is oxidized to NAD and is measured photometrically by a decrease in absorbance. For each mole of substrate hydrolyzed, 2 moles of coenzyme (NADH) are oxidized. This 1:2 ratio is applied in the calculation of aldolase activity. Lactate dehydrogenase (LDH) is present in the reagent for the purpose of eliminating interference from endogenous pyruvate in the specimen.(Package insert: Roche Aldolase Reagent. Roche Diagnostics; V 4.0, 05/2017)
Report Available
1 to 3 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
Method Name
Photometric
Forms
If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.