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Test Code ALMIT Mitochondrial Antibodies (M2), Serum

Reporting Name

Mitochondrial Ab, M2, S

Useful For

Establishing the diagnosis of primary biliary cirrhosis

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type


Specimen Required


Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL

Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 21 days
  Frozen  21 days

Reference Values

Negative: <0.1 Units

Borderline: 0.1-0.3 Units

Weakly positive: 0.4-0.9 Units

Positive: ≥1.0 Units

Reference values apply to all ages.

Day(s) and Time(s) Performed

Monday through Saturday; 11 a.m.

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
AMA Mitochondrial Ab, M2, S 51715-1


Result ID Test Result Name Result LOINC Value
AMA Mitochondrial Ab, M2, S 51715-1

Clinical Reference

1. Rich R, Fleisher T, Shearer W, et al: Inflammatory hepatobiliary cirrhosis. In Clinical Immunology Principles and Practice. Third edition. Philadelphia, Elsevier 2008 April 15

2. Muratori L, Granito A, Muratori P, et al: Antimitochondrial antibodies and other antibodies in primary biliary cirrhosis: diagnostic and prognostic value. Clin Liver Dis 2008;12:261-276

3. Kaplan MM, Gershwin ME: Primary biliary cirrhosis. N Engl J Med 2005;353(12):1261-1273

4. Van Norstrand MD, Malinchoc M, Lindor KD, et al: Quantitative measurement of autoantibodies to recombinant mitochondrial antigens in patients with primary biliary cirrhosis: relationship to levels of autoantibodies to disease progression. Hepatology 1997;25(1):6-11

Method Description

Enzyme immunosorbent assay with purified M2 antigens. This method detects both IgG and IgM antibodies to M2 antigens. Testing is performed on the Agility instrument by Dynex.(Package insert: Bio-Rad, Kallestad Anti-Mitochondrial Kit, Distributed by Bio-Rad Laboratories, Inc., Hercules, CA 04/14)

Analytic Time

1 day

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus OK

Method Name

Enzyme Immunoassay (EIA)


If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-General Request (T239)

-Gastroenterology and Hepatology Client Test Request (T728)

Secondary ID