Home
HelpTest Code ANIDE Organism Referred for Identification, Anaerobic Bacteria
Infectious
Reporting Name
Organism Ref for ID, Anaerobic BactUseful For
Identification of anaerobic bacteria involved in human infections
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
VariesOrdering Guidance
If susceptibility testing is needed; order MMLSA / Antimicrobial Susceptibility, Anaerobic Bacteria, MIC also. If susceptibilities are not appropriate, MMLSA will be canceled at report time.
Shipping Instructions
1. See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.
Necessary Information
1. Specimen source is required.
2. Isolate description is required: Gram stain reaction, morphology, tests performed.
Specimen Required
Supplies:
Anaerobic Transport Tube (T588)
Thioglycollate broth or any other suitable anaerobic transport system
Infectious Container, Large (T146)
Specimen Type: Pure culture of organism from a source not normally colonized by anaerobes
Acceptable Sources: Abscesses, percutaneous transtracheal aspirates, sterile body fluids, suprapubic aspirations, or wounds
Container/Tube:
Preferred: Anaerobic transport tube (T588)
Acceptable: Thioglycollate broth or any other suitable anaerobic transport system
Collection Instructions:
1. Perform isolation of infecting bacteria.
2. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures.
Specimen Minimum Volume
NA
Testing Algorithm
When this test is ordered, the reflex tests may be performed and charged. All bacterial organisms submitted will be identified and billed as appropriate.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient (preferred) | |
| Refrigerated | ||
Special Instructions
Reference Values
Identification of organism
Day(s) Performed
Monday through Sunday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
87076-Organism ref for ID, anaerobic bact
87076-Id MALDI-TOF mass spec anaerobe (if appropriate)
87076-Anaerobe Ident (if appropriate)
87153-Anaerobe ident by sequencing (if appropriate)
87077-Identification commercial kit (if appropriate)
87077-Ident by MALDI-TOF mass spec (if appropriate)
87077-Bacteria identification (if appropriate)
87077-Additional identification procedure (if appropriate)
87077-Identification Staphylococcus (if appropriate)
87077-Identification Streptococcus (if appropriate)
87147 x 3-Serologic agglut method 1 ident (if appropriate)
87147-Serologic agglut method 2 ident (if appropriate)
87147 x 4-Serologic agglut method 3 ident (if appropriate)
87147 x 2-6 - Serologic Agglut Method 4 Ident (if appropriate)
87153-Aerobe ident by sequencing (if appropriate)
87798-Identification by PCR (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ANIDE | Organism Ref for ID, Anaerobic Bact | 20878-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| ANIDE | Organism Ref for ID, Anaerobic Bact | 20878-5 |
Clinical Reference
1. Jousimies-Somer HR, Summanen P, Citron DM, et al: Wadsworth Anaerobic Bacteriology Manual. Sixth edition. Belmont, CA, Star Publishing Co. 2002
2. Anaerobic Bacteria. Chapters 50-54. In Manual of Clinical Microbiology. 11th edition. Edited by JH Jorgensen, MA Pfaller. Washington, DC, ASM Press, 2015
3. Hall GS: Section 4. Anaerobic bacteriology. In Clinical Microbiology Procedures Handbook. Vol 1. Third edition. Edited by LS Garcia. Washington, DC, ASM Press, 2010
Method Description
Appropriately transported organisms are inoculated onto blood agar plates and into thioglycollate broth tubes. After 48 hours of incubation at 35° C in an anaerobic atmosphere, colonies are identified using one or a combination of the following techniques: Gram stain, use of various differential media, aero tolerance testing, conventional biochemical tests, MALDI-TOF mass spectrometry, or 16S ribosomal RNA gene sequencing.(Koneman EW: The anaerobic bacteria. In Color Atlas and Textbook of Diagnostic Microbiology, Sixth edition. Philadelphia, Lippincott, Williams and Wilke, 2006, pp 877-944)
Report Available
8 to 14 daysReject Due To
| Other | Agar plate |
Method Name
Dependent on organism submitted, 1 or more of the following methods will be used: media, aero tolerance testing, conventional biochemical tests (MALDI-TOF) mass spectrometry or 16S RNA gene sequencing
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| RMALA | Id MALDI-TOF Mass Spec Anaerobe | No, (Bill Only) | No |
| ANAID | Anaerobe Ident | No, (Bill Only) | No |
| ISAN | Anaerobe Ident by Sequencing | No, (Bill Only) | No |
| COMM | Identification Commercial Kit | No, (Bill Only) | No |
| RMALD | Ident by MALDI-TOF mass spec | No, (Bill Only) | No |
| GID | Bacteria Identification | No, (Bill Only) | No |
| ISAE | Aerobe Ident by Sequencing | No, (Bill Only) | No |
| REFID | Additional Identification Procedure | No, (Bill Only) | No |
| SALS | Serologic Agglut Method 1 Ident | No, (Bill Only) | No |
| EC | Serologic Agglut Method 2 Ident | No, (Bill Only) | No |
| SHIG | Serologic Agglut Method 3 Ident | No, (Bill Only) | No |
| STAP | Identification Staphylococcus | No, (Bill Only) | No |
| STRP | Identification Streptococcus | No, (Bill Only) | No |
| SIDC | Ident Serologic Agglut Method 4 | No, (Bill Only) | No |
| PCRID | Identification by PCR | No, (Bill Only) | No |