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Test Code CEAPT Carcinoembryonic Antigen (CEA), Peritoneal Fluid

Reporting Name

CEA, Peritoneal Fluid

Useful For

An adjunct to cytology to differentiate between malignancy-related and benign causes of ascites formation

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type


Specimen Required

Container/Tube:  Plain, plastic, screw top tube

Specimen Volume: 2 mL

Specimen Minimum Volume

0.5 mL (Samples <0.5 mL may be rejected)

Specimen Stability Information

Specimen Type Temperature Time Special Container
Peritoneal Frozen (preferred) 90 days
  Ambient  7 days
  Refrigerated  7 days

Reference Values

An interpretive report will be provided.

Day(s) and Time(s) Performed

Monday through Friday; Varies

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
CEAPT CEA, Peritoneal Fluid 40622-3


Result ID Test Result Name Result LOINC Value
CEAPN CEA, Peritoneal Fluid 40622-3
SITED Site 39111-0

Clinical Reference

1. Torresini RJ, Prolla JC, Diehl AR, et al: Combined carcinoembryonic antigen and cytopathologic examination in ascites. Acta Cytol 2000;44(5):778-782

2. Tuzun Y, Yilmaz S, Dursun M, et al: How to increase the diagnostic value of malignancy-related ascites: discriminative ability of the ascitic tumour markers. J Int Med Res 2009;37(1):87-95

Method Description

The instrument used is Beckman Coulter UniCel DXI 800. The Access CEA assay is a 2-site immunoenzymatic sandwich assay using mouse monoclonal anti-carcinoembryonic antigen (CEA) antibodies that react with different epitopes of CEA. A sample is added to a reaction vessel, along with the first anti-CEA monoclonal antibodies-alkaline phosphatase conjugate and the second anti-CEA monoclonal antibodies bound to paramagnetic particles. The incubation is followed by a magnetic separation and washing. The chemiluminescent substrate Lumi-Phos 530 is added to the vessel and light generated by the reaction is measured with a luminometer. The light production is proportional to the concentration of CEA in the sample. The amount of analyte in the sample is determined by means of a stored, multipoint calibrator curve.(Package insert: Access CEA Assay, Beckman Coulter, Inc, Fullerton, CA, 2007)


For all samples with CEA concentrations >3 ng/mL, a dilution series is performed. A linear dilution excludes hooking and most major interferences. Samples that contain CEA concentrations ≤3 ng/mL are spiked with exogenous CEA to identify possible interferences that may cause a false-low result.

Analytic Time

Same day/1 day

Reject Due To

Gross hemolysis Reject

Method Name

Immunoenzymatic Assay