Test Code CXURN Urine Culture
Specimen Required
•Grey Top Urine Transport Container
•Sterile Container (only acceptable if the minimum fill on the Grey Top tube cannot be reached)
Specimen Minimum Volume
Fill line on grey top urine transport container
Report Available
Initial report available at one day after receipt in the laboratory, final report depends on the extent of organism workup
Clinical Significance
Isolation of a pathogen may indicate an infectious cause of symptoms. Three or more species of bacteria isolated in a urine specimen usually indicates skin or fecal contamination at the time of collection and the laboratory report will suggest recollection.
Specimen Stability Information
• Grey Top Must be received within 48hrs of collection •Refrigerated urine in sterile container must be received within 24 hours of collection
Specimen Instructions
Clean Catch: Clean genitals with towelette, collect midstream urine Indwelling Catheter: Collect from sampling port of a newly inserted device In and Out Catheter: Clean area, using sterile technique, collect and discard an initial 15-30 ml of urine. Collect a sample from the mid- or later flow of urine into a sterile container 1) Collect urine into sterile collection container and then transfer urine to a Grey Top urine transport tube
For BD VACUTAINER URINE COMPLETE CUP KIT (follow product insert)
a. Collect urine into the blue capped cup.
b. To transfer specimen, place cup on a clean, flat surface.
c. Peel back label on cap to expose the integrated transfer device.
d. Place evacuated tube into cavity on cap, stopper down. Advance the tube over the puncture point to pierce stopper.
e. Hold tube in position until filled.
f. Remove tube from integrated transfer device.
g. Mix 8-10 times by inversion.
2) Apply patient identification label.
3) Submit Grey Top tube to the laboratory
SETUP SCHEDULE
Daily
INCLUDES
Aerobic culture of urine specimens for detection of bacteria and yeast
TRANSPORT/STORAGE CONDITION
Refrigerated
Clinical Reference
Negative for Pathogens
Method Description
Quantitative (reported in CFU/ml) aerobic bacterial and yeast culture
Reject Due To
Urine must be refrigerated or placed in a Urine Transport Media tube within 30 minutes of collection, urine collected from catheter bags is unacceptable. Urine in sterile container received at room temperature is unacceptable.
Category
Culture