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Test Code DAGR Dairy and Grain Allergen Profile, Serum

Reporting Name

Dairy and Grain Allergen Profile

Useful For

Establishing a diagnosis of an allergy to dairy and grain

 

Defining the allergen responsible for eliciting signs and symptoms

 

Identifying allergens:

-Responsible for allergic disease and/or anaphylactic episode

-To confirm sensitization prior to beginning immunotherapy

-To investigate the specificity of allergic reactions to insect venom allergens, drugs, or chemical allergens

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Advisory Information


For a listing of allergens available for testing, see Allergens - Immunoglobulin E (IgE) Antibodies in Special Instructions



Specimen Required


Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Specimen Volume: 0.7 mL


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  90 days

Reference Values

Class

IgE kU/L

Interpretation

0

<0.35

Negative

1

0.35-0.69

Equivocal

2

0.70-3.49

Positive

3

3.50-17.4

Positive

4

17.5-49.9

Strongly positive

5

50.0-99.9

Strongly positive

6

≥100

Strongly positive

Reference values apply to all ages.

Day(s) and Time(s) Performed

Monday through Friday; 9 a.m.-8 p.m.,

Saturday; 8 a.m.-3 p.m.

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86003 x 5

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DAGR Dairy and Grain Allergen Profile 49222-3

 

Result ID Test Result Name Result LOINC Value
EGG Egg White, IgE 6106-9
MILK Milk, IgE 6174-7
OATS Oat, IgE 6190-3
SOY Soybean, IgE 6248-9
WHT Wheat, IgE 6276-0

Clinical Reference

Homburger HA, Hamilton RG: Chapter 55: Allergic diseases. In Henry's Clinical Diagnosis and Management by Laboratory Methods. 23rd edition. Edited by RA McPherson, MR Pincus. Elsevier, 2017, pp 1057-1070

Method Description

Specific IgE from the patient's serum reacts with the allergen of interest, which is covalently coupled to an ImmunoCAP. After washing away nonspecific IgE, enzyme-labeled anti-IgE antibody is added to form a complex. After incubation, unbound anti-IgE is washed away and the bound complex is then incubated with a developing agent. After stopping the reaction, the fluorescence of the eluate is measured. Fluorescence is proportional to the amount of specific IgE present in the patient's sample (ie, the higher the fluorescence value, the more IgE antibody is present).(Package insert: ImmunoCAP System Specific IgE FEIA, Uppsala, Sweden Rev 06/2019)

Analytic Time

Same day/1 day

Reject Due To

Gross hemolysis OK
Gross lipemia OK

Profile Information

Test ID Reporting Name Available Separately Always Performed
EGG Egg White, IgE Yes Yes
MILK Milk, IgE Yes Yes
WHT Wheat, IgE Yes Yes
OATS Oat, IgE Yes Yes
SOY Soybean, IgE Yes Yes