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Test Code DGLDN Gliadin (Deamidated) Antibodies Evaluation, IgG and IgA, Serum

Reporting Name

Gliadin (Deamidated) Ab, Eval, S

Useful For

Evaluating patients suspected of having celiac disease; this includes patients with symptoms compatible with celiac disease, patients with atypical symptoms, and individuals at increased risk of celiac disease


Evaluating the response to treatment with a gluten-free diet

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type


Advisory Information

Cascade testing is recommended for celiac disease. Cascade testing ensures that testing proceeds in an algorithmic fashion. The following cascades are available; select the appropriate one for your specific patient situation.

-CDCOM / Celiac Disease Comprehensive Cascade: complete testing including HLA DQ

-CDSP / Celiac Disease Serology Cascade: complete testing excluding HLA DQ

-CDGF / Celiac Disease Gluten-Free Cascade: for patients already adhering to a gluten-free diet

To order individual tests, see Celiac Disease Diagnostic Testing Algorithm in Special Instructions.

Specimen Required


Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL

Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 21 days
  Frozen  21 days

Reference Values

Negative: <20.0 U

Weak positive: 20.0-30.0 U

Positive: >30.0 U

Reference values apply to all ages.

Day(s) and Time(s) Performed

Monday through Saturday; 4 p.m.

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

83516 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DGLDN Gliadin (Deamidated) Ab, Eval, S 57776-7


Result ID Test Result Name Result LOINC Value
DAGL Gliadin(Deamidated) Ab, IgA, S 47393-4
DGGL Gliadin(Deamidated) Ab, IgG, S 47394-2

Clinical Reference

1. Green PH, Cellier C: Celiac disease. New Engl J Med 2007;357:1731-1743

2. Green PH, Jabri B: Celiac disease. Annu Rev Med 2006;57:207-221

3. Harrison MS, Wehbi M, Obideen K: Celiac disease: More common than you think. Cleve Clin J Med 2007;74:209-215

4. Dale JC, Homburger HA, Masoner DE, et al: Update on celiac disease: New standards and new tests. Mayo Communique 2008;33(6):1-9

5. Rashtak S, Ettore MW, Homburger HA, et al: Comparative usefulness of deamidated gliadin antibody measurements in the diagnosis of celiac disease. Clin Gastroenterol Hepatol 2008 Apr;6(4):426-432

6. Sugai E, Vazquez H, Nachman F, et al: Accuracy of testing for antibodies to synthetic gliadin-related peptides in celiac disease. Clin Gastroenterol Hepatol 2006;4:1112-1117

Method Description

IgA and IgG antibodies to deamidated gliadin peptides are detected by enzyme-linked immunosorbent assay by binding to purified peptides adsorbed to the wells of a microtiter plate. Peptides are bound to the wells under conditions that preserve the antigens in their native states. Prediluted controls and diluted patient sera are added to separate wells allowing antibodies to bind to the deamidated gliadin peptides. Unbound sample constituents are washed away and horseradish peroxidase-labeled antihuman IgA or IgG antibody conjugate is added to each well. After a second incubation, unbound enzyme-labeled conjugate is washed away and bound conjugate is detected by adding tetramethylbenzidine chromogenic substrate. After a final incubation, colored product is measured spectrophotometrically and the absorbance compared to the low, positive calibrator. The intensity of color is directly proportional to the level of IgA or IgG antibodies to deamidated gliadin peptides expressed in arbitrary units.(Instruction manual: QUANTA Lite Gliadin IgA II (rev. 1, 4/2015). INOVA Diagnostics, Inc. San Diego, CA, 92131; Instruction manual: QUANTA Lite Gliadin IgG II (rev. 3, 4/2017). INOVA Diagnostics, Inc. San Diego, CA, 92131)

Analytic Time

1 day

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus OK

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)


If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Client Test Request (T728) with the specimen.

Profile Information

Test ID Reporting Name Available Separately Always Performed
DAGL Gliadin(Deamidated) Ab, IgA, S Yes Yes
DGGL Gliadin(Deamidated) Ab, IgG, S Yes Yes

Secondary ID