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Test Code DHEAS Dehydroepiandrosterone Sulfate, Serum

Additional Codes

Epic: LAB524

Mayo: DHES1

Reporting Name

Dehydroepiandrosterone Sulfate, S

Useful For

Diagnosis and differential diagnosis of hyperandrogenism (in conjunction with measurements of other sex steroids)

 

An adjunct in the diagnosis of congenital adrenal hyperplasia

 

Diagnosis and differential diagnosis of premature adrenarche

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.6 mL


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  30 days

Reference Values

MALES

1-14 days: DHEA-S levels in newborns are very elevated at birth but will fall to prepubertal levels within a few days.

Tanner Stages* 

Mean

Age

Reference Range (mcg/dL)

Stage I

>14 days

11-120

Stage II

11.5 years

14-323

Stage III

13.6 years

5.5-312

Stage IV

15.1 years

29-412

Stage V

18.0 years

104-468

*Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for boys at a median age of 11.5 (±) 2 years. For boys, there is no proven relationship between puberty onset and body weight or ethnic origin. Progression through Tanner stages is variable. Tanner stage V (adult) is usually reached by age 18.

18-30 years: 105-728 mcg/dL

31-40 years: 57-522 mcg/dL

41-50 years: 34-395 mcg/dL

51-60 years: 20-299mcg/dL

61-70 years: 12-227 mcg/dL

≥71 years: 6.6-162 mcg/dL

FEMALES

1-14 days: DHEA-S levels in newborns are very elevated at birth but fall to prepubertal levels within a few days.

Tanner Stages* 

Mean

Age

Reference Range (mcg/dL)

Stage I

>14 days

16-96

Stage II

10.5 years

22-184

Stage III

11.6 years

11-296

Stage IV

12.3 years

17-343

Stage V

14.5 years

57-395

*Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for girls at a median age of 10.5 (±) 2 years. There is evidence that it may occur up to 1 year earlier in obese girls and in African American girls. Progression through Tanner stages is variable. Tanner stage V (adult) is usually reached by age 18.

18-30 years: 83-377 mcg/dL

31-40 years: 45-295 mcg/dL

41-50 years: 27-240 mcg/dL

51-60 years: 16-195 mcg/dL

61-70 years: 9.7-159

≥71 years: 5.3-124 mcg/dL

Day(s) and Time(s) Performed

Monday through Friday; 6 a.m.-12 a.m.

Saturday; 6 a.m.-6 p.m.

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

82627

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DHES1 Dehydroepiandrosterone Sulfate, S 2191-5

 

Result ID Test Result Name Result LOINC Value
DHES1 Dehydroepiandrosterone Sulfate, S 2191-5

Clinical Reference

1. Kricka L. Interferences in immunoassays - still a threat. Clin Chem 2000;46:1037-1038

2. Bjerner J, et al. Immunometric assay interference: incidence and prevention. Clin Chem 2002;48:613-621

3. Sciarra F, Tosti-Croce C, Toscano V: Androgen-secreting adrenal tumors. Minerva Endocrinol 1995;20:63-68

4. Young WF Jr: Management approaches to adrenal incidentalomas-a view from Rochester, Minnesota. Endocrinol Metab Clin North Am 2000;21:671-696

5. Ibanez L, DiMartino-Nardi J, Potau N, Saenger P: Premature adrenarche-normal variant or forerunner of adult disease? Endocrine Reviews 2001;40:1-16

6. Collett-Solberg P: Congenital adrenal hyperplasia: from genetics and biochemistry to clinical practice, part I. Clin Pediatr 2001;40:1-16

7. Allolio B, Arlt W: DHEA treatment: myth or reality? Trends Endocrinol Metab 2002;13:288-294

8. Salek FS, Bigos KL, Kroboth PD: The influence of hormones and pharmaceutical agents on DHEA and DHEA-S concentrations: a review of clinical studies. J Clin Pharmacol 2002;42:247-266

9. Elmlinger MW, Kuhnel W, Ranke MB: Reference ranges for serum concentrations of lutropin (LH), follitropin (FSH), estradiol (E2), prolactin, progesterone, sex hormone binding globulin (SHBG), dehydroepiandrosterone sulfate (DHEA-S), cortisol and ferritin in neonates, children, and young adults. Clin Chem Lab Med 2002;40(11):1151-1160

Method Description

The Access DHEA-S assay is a competitive binding immunoenzymatic assay. A sample is added to a reaction vessel with paramagnetic particles coated with goat antirabbit:rabbit anti-DHEA-S and DHEA-S alkaline phosphatase conjugate in TRIS-buffered protein solution. After incubation in a reaction vessel, materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the chemiluminescent substrate Lumi-Phos* 530 is added to the vessel and light generated by the reaction is measured with a luminometer. The light production is inversely proportional to the concentration of DHEA-S in the sample. The amount of analyte in the sample is determined from a stored, multipoint calibration curve.(Package insert:Beckman Coulter Inc, Fullerton, CA 2017)

Analytic Time

Same day/1 day

Reject Due To

Gross hemolysis Reject
Gross lipemia OK

Method Name

Immunoenzymatic Assay

Forms

If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.

Secondary ID

113595