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Test Code DSAC Disaccharidase Activity Panel, Tissue

Shipping Instructions

Freeze immediately after collection and ship frozen.

Specimen Required

Specimen Type: Tissue

Source: Intestinal biopsy

Container/Tube: Clean, screw-topped plastic vial

Specimen Volume: 5 mg

Collection Instructions: Specimen should not be placed on gauze or filter paper, nor should any saline, support or embedding material be added.

Secondary ID


Useful For

Evaluation of patients who present with signs or symptoms suggestive of disaccharidase disorders

This test is not intended for carrier detection.

Method Name


Reporting Name

Disaccharidase Activity Panel, Ts

Specimen Type


Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Tissue Frozen 28 days OTHER

Reject Due To

Specimens sent in formaldehyde, saline, or any supportive media
Filter paper

Reference Values

Lactase: ≥14.0 nmol/min/mg protein

Sucrase: ≥19.0 nmol/min/mg protein

Maltase: ≥70.0 nmol/min/mg protein

Palatinase: ≥6.0 nmol/min/mg protein

Glucoamylase: ≥8.0 nmol/min/mg protein

Clinical Reference

1. Cohen SA, Oloyede H, Gold BD, Mohammed A, Elser HE: Clinical characteristics of disaccharidase deficiencies among children undergoing upper endoscopy. J Pediatr Gastroenterol Nutr. 2018 Jun;66 Suppl 3:S56-S60

2. Semenza G, Auricchio S, Mantei N. : Small-intestinal disaccharidases. In: Valle DL, Antonarakis S, Ballabio A, Beaudet AL, Mitchell GA. eds. The Online Metabolic and Molecular Bases of Inherited Disease. McGraw Hill; 2019. Accessed August 23, 2021. Available at

Method Description

Disaccharidase activities are measured by incubating intestinal homogenates with an appropriate disaccharide substrate, which releases glucose. Glucose production is determined by a glucose oxidase reagent mixture that oxidizes glucose to produce peroxide, which reacts with phenol aminophenazone to form a red quinoneimine dye. The concentration of quinoneimine is determined spectrophotometrically and the concentration of liberated glucose is calculated from a glucose standard curve. The amount of glucose produced is proportional to the disaccharidase activity in the intestinal homogenate. Disaccharidase activities are expressed as nanomoles of glucose per minute per milligram of protein (nmol/min/mg prot).(Unpublished Mayo method)

Day(s) Performed

Monday through Friday

Report Available

3 to 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
DSAC Disaccharidase Activity Panel, Ts 57765-0


Result ID Test Result Name Result LOINC Value
608237 Lactase 81315-4
608238 Sucrase 30372-7
608239 Maltase 30315-6
608240 Palatinase 81314-7
608241 Glucoamylase 81322-0
608242 Interpretation 57779-1
608243 Reviewed By 18771-6