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HelpTest Code EBVE Epstein-Barr Virus (EBV), IgG Antibody to Early Antigen, Serum
Reporting Name
EBV EA IgG Ab, SUseful For
A third-order test in the diagnosis of infectious mononucleosis, especially in situations when initial testing results (heterophile antibody test) are negative and follow-up testing (viral capsid antigen: VCA IgG, VCA IgM, and Epstein-Barr nuclear antigen) yields inconclusive results aiding in the diagnosis of type 2 or type 3 nasopharyngeal carcinoma
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumSpecimen Required
Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Specimen Volume: 1 mL
Specimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 14 days |
Reference Values
Negative
Reference values apply to all ages.
Day(s) and Time(s) Performed
Monday through Saturday; 9 a.m.
Test Classification
This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86663
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| EBVE | EBV EA IgG Ab, S | 22295-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| EBVE | EBV EA IgG Ab, S | 22295-0 |
Clinical Reference
1. Fields BN, Knipe DM: Epstein-Barr virus. In: Fields BN, Knipe DM, Howley PM, eds. Fields Virology. 4th ed. Lippincott Williams and Wilkins; 2001
2. Lennette ET: Epstein-Barr virus. In: Murray PR, Baron EJ, Pfaller MA, et al, eds. Manual of Clinical Microbiology. 6th ed. ASM Press; 1995:905-910
Method Description
Testing is performed on the BioPlex 2200 System for the detection of the early antigen-diffuse (EA-D) antibody. An aliquot of the patient serum, sample diluent, and bead reagent are combined in a reaction vessel. After washing, antihuman-IgG antibody conjugated to phycoerythrin (PE) is added to the beads and incubated. Another wash step removes excess conjugate, and beads are subsequently resuspended in wash buffer. The bead mixture passes through a detector where the identity of each bead is determined by the bead's dye fluorescence. In addition, the amount of antibody captured by the antigen is measured by the fluorescence of the bound PE.(Package insert: BioPlex 2200 System EBV EA-D. Bio-Rad Laboratories; 2007)
Analytic Time
Same day/1 dayReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Heat-activated specimen | Reject |
Method Name
Multiplex Flow Immunoassay
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.