Sign in →

Test Code EBVE Epstein-Barr Virus (EBV), IgG Antibody to Early Antigen, Serum

Reporting Name

EBV EA IgG Ab, S

Useful For

A third-order test in the diagnosis of infectious mononucleosis, especially in situations when initial testing results (heterophile antibody test) are negative and follow-up testing (viral capsid antigen: VCA IgG, VCA IgM, and Epstein-Barr nuclear antigen) yields inconclusive results aiding in the diagnosis of type 2 or type 3 nasopharyngeal carcinoma

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 1 mL


Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reference Values

Negative

Reference values apply to all ages.

Day(s) and Time(s) Performed

Monday through Saturday; 9 a.m.

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86663

LOINC Code Information

Test ID Test Order Name Order LOINC Value
EBVE EBV EA IgG Ab, S 22295-0

 

Result ID Test Result Name Result LOINC Value
EBVE EBV EA IgG Ab, S 22295-0

Clinical Reference

1. Fields BN, Knipe DM: Epstein-Barr virus. In: Fields BN, Knipe DM, Howley PM, eds. Fields Virology. 4th ed. Lippincott Williams and Wilkins; 2001

2. Lennette ET: Epstein-Barr virus. In: Murray PR, Baron EJ, Pfaller MA, et al, eds. Manual of Clinical Microbiology. 6th ed. ASM Press; 1995:905-910

Method Description

Testing is performed on the BioPlex 2200 System for the detection of the early antigen-diffuse (EA-D) antibody. An aliquot of the patient serum, sample diluent, and bead reagent are combined in a reaction vessel. After washing, antihuman-IgG antibody conjugated to phycoerythrin (PE) is added to the beads and incubated. Another wash step removes excess conjugate, and beads are subsequently resuspended in wash buffer. The bead mixture passes through a detector where the identity of each bead is determined by the bead's dye fluorescence. In addition, the amount of antibody captured by the antigen is measured by the fluorescence of the bound PE.(Package insert: BioPlex 2200 System EBV EA-D. Bio-Rad Laboratories; 2007)

Analytic Time

Same day/1 day

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Heat-activated specimen Reject

Method Name

Multiplex Flow Immunoassay

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.