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Test Code EBVQP Epstein-Barr Virus DNA, Quantitative Real-Time PCR

Additional Codes

Epic: LAB12449

Quest: 10186

Specimen Required

Specimen Type: Whole Blood EDTA
Collection Container/Tube: 
Preferred: Lavender top (EDTA)
Acceptable: ACD solution B (yellow-top) tube
Specimen Volume: 1.0 mL

 

Specimen Type: Plasma
Collection Container/Tube
Preferred: PPT-potassium EDTA (white-top) tube
Acceptable: Lavender top (EDTA), ACD solution B (yellow-top) tube, 
Submission Container/Tube: Plastic vial
Specimen Volume: 1.0 mL

 

Specimen Type: CSF
Collection Container/Tube: Sterile container
Submission Container/Tube: Plastic vial
Specimen Volume: 1.0 mL
Collection Instructions: Collect in a sterile, plastic container and store refrigerated or frozen.

 

Specimen Type: Bone Marrow
Collection Container/Tube
Preferred: Lavender top (EDTA)
Acceptable: ACD solution B (yellow-top) tube

 

Specimen Type: Serum
Collection Container/Tube: Serum gel
Submission Container/Tube: Plastic vial
Specimen Volume: 1.0 mL
Collection Instructions: Centrifuge and separate cells after clot formation and within 4 hours of collection.

 

Specimen Type: Bronchoalveolar lavage or Eye fluid
Collection Container/Tube: Sterile container
Specimen Volume: 1.0 mL

Specimen Minimum Volume

0.5 mL

Specimen Stability

Whole blood, bone marrow
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable

All other specimens
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: 30 days

Clinical Significance

Monitoring EBV DNA levels by quantitative PCR in patients at risk of EBV-associated lymphoproliferative disorders may allow timely recognition of virus reactivation and permit installment of antiviral treatment. This is a quantitative molecular test, with a linear range of 200-2,000,000 copies/mL.

Reference Range

EBV DNA, QN PCR: <200 copies/mL

EBV DNA, QN PCR: <2.30 Log copies/mL

Rejection Due To

Heparin anticoagulant, Thawed specimen, Avoid repeated freezing and thawing
 

Day(s) and Time(s) Performed

Daily

Report Available

1 day

Method Name

Real-Time Polymerase Chain Reaction

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

CPT Codes

10186