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Test Code EBVQU Epstein-Barr Virus DNA Detection and Quantification, Plasma

Reporting Name

EBV DNA Detect / Quant, P

Useful For

Diagnosis of EBV-associated infectious mononucleosis in individuals with equivocal or discordant Epstein-Barr virus (EBV) serologic marker test results


Diagnosis of posttransplant lymphoproliferative disorders (PTLD), especially in EBV-seronegative organ transplant recipients receiving antilymphocyte globulin for induction immunosuppression and OKT-3 treatment for early organ rejection


Monitoring progression of EBV-associated PTLD in organ transplant recipients

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Plasma EDTA

Shipping Instructions

1. Freeze plasma specimen immediately, and ship specimen frozen on dry ice.

2. If shipment will be delayed for more than 7 days, freeze plasma specimen at -20° C (up to 30 days) until shipment on dry ice.

Specimen Required

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions:

1. Centrifuge blood collection tube per collection tube manufacturer's instructions.

2. Pour off plasma into aliquot tube.

Specimen Minimum Volume

0.8 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma EDTA Frozen (preferred) 30 days
  Refrigerated  7 days

Reference Values


Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
EBVQU EBV DNA Detect / Quant, P 43730-1


Result ID Test Result Name Result LOINC Value
65754 EBV DNA Detect / Quant, P 43730-1

Clinical Reference

1. Kimura H, Ito Y, Suzuki R, et al: Measuring Epstein-Barr virus (EBV) load: the significance and application for each EBV-associated disease. Rev Med Virol 2008;18(5):305-319

2. Gulley ML, Tang W: Using Epstein-Barr viral load assays to diagnose, monitor, and prevent post-transplant lymphoproliferative disorder. Clin Microbiol Rev 2010;23(2):350-366

3. Ruf S, Wagner HJ: Determining EBV load: current best practice and future requirements. Expert Rev Clin Immunol 2013;9(2):139-151

4. San-Juan R, Comoli P, Caillard S, et al: Epstein-Barr virus-related post-transplant lymphoproliferative disorder in solid organ transplant recipients. Clin Microbiol Infect 2014;20(Suppl 7):109-118

5. Jiang SY, Yang JW, Shao JB, et al: Real-time polymerase chain reaction for diagnosing infectious mononucleosis in pediatric patients: a systematic review and meta-analysis. J Med Virol 2016;88(5):871-876.   

Method Description

Testing is performed using a combination of MGB Alert EBV Primer and Probe ASRs (ELITechGroup Molecular Diagnostics, Inc.) and 2x MGB Alert PCR Master Mix with the Abbott m2000 RealTime System (Abbott Molecular, Inc.). The MGB Alert reagents are designed based on minor groove-binding Pleiades probe chemistry. This assay amplifies the p140 region of the BNRF1 gene of Epstein-Barr virus (EBV) for detection and quantification of EBV DNA in human plasma.


The Abbott mSample Preparation System kit is used with the automated Abbott m2000sp sample preparation system to extract and purify viral DNA from human plasma specimens, based on magnetic particle technology. An internal control template is introduced into each specimen during sample preparation to assess extraction failure or inhibition during the sample preparation and qPCR processes, respectively. Amplification and detection of target sequence is performed on the Abbott m2000rt instrument. The assay is calibrated to the First World Health Organization International Standard for EBV, NIBSC code: 09/260 by using commercially available EBV verification panels consisting of multiple panel members calibrated to the WHO standard and with EBV DNA levels ranging over 4 log IU/mL.(Unpublished Mayo method)

Reject Due To

Gross hemolysis OK
Gross lipemia OK

Method Name

Real-Time Polymerase Chain Reaction (PCR) Followed by Minor Groove-Binding (MGB) Probe Hybridization


If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-General Request (T239)

-Microbiology Test Request (T244)

-Renal Diagnostics Test Request (T830)

Secondary ID