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Test Code EQUIN Western Equine Encephalitis Antibody, IgG and IgM, Serum

Reporting Name

West Equine Enceph Ab,IgG and IgM,S

Useful For

Aiding the diagnosis of Western equine encephalitis

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL


Specimen Minimum Volume

0.15 mL

Testing Algorithm

See Mosquito-borne Disease Laboratory Testing in Special Instructions.

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reference Values

IgG: <1:10

IgM: <1:10

Reference values apply to all ages.

Day(s) and Time(s) Performed

May through October: Monday through Friday; 9 a.m.

November through April: Monday, Wednesday, Friday; 9 a.m.

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86654 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
WEEP West Equine Enceph Ab,IgG and IgM,S 69041-2

 

Result ID Test Result Name Result LOINC Value
8193 West Equine Enceph Ab, IgG, S 6957-5
87279 West Equine Enceph Ab, IgM, S 6958-3

Clinical Reference

1. Gonzalez-Scarano F, Nathanson N: Bunyaviruses. In: Fields BN, Knipe DM, eds. Fields Virology. Vol 1. 2nd ed. Raven Press; 1990:1195-1228

2. Donat JF, Hable-Rhodes KH, Groover RV, Smith TF: Etiology and outcome in 42 children with acute nonbacterial meningoencephalitis. Mayo Clin Proc. 1980;55:156-160

3. Tsai TF: Arboviruses. In: Murray PR, Baron EJ, Pfaller MA, et al, eds. Manual of Clinical Microbiology. 7th ed. American Society for Microbiology; 1999:1107-1124

4. Calisher CH: Medically important arboviruses of the United States and Canada. Clin Microbiol Rev. 1994;7:89-116

5. Markoff L: Alphaviruses (Chikungunya, Eastern equine encephalitis). In: Bennett JE, Dolin R, Blaser MJ, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 9th ed. Elsevier; 2020:1997-2006

Method Description

Indirect immunofluorescence. Dilutions of test sera are prepared and allowed to react with substrate cells infected with Western equine encephalitis virus. If IgG antibodies to this virus are present in the serum of the patient, an antigen-antibody complex will develop that can be detected by a fluorescein-labeled antibody directed to human globulins.(Tsai TF: Arboviruses. In: Murray PR, Baron EJ, Pfaller MA, et al, eds. Manual of Clinical Microbiology. 7th ed. American Society for Microbiology; 1999:1107-1124; Beaty BJ, Casals J, Brown KL, et al: Indirect fluorescent-antibody technique for serological diagnosis of LaCrosse [California] virus infections. J Clin Microbiol. 1982;15:429-434; Beckham JD, Tyler KL: Arbovirus Infections. Continuum (Minneap Minn). 2015 Dec;21(6 Neuroinfectious Disease):1599-1611. doi: 10.1212/CON.0000000000000240)

Analytic Time

Same day/1 day

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Method Name

Immunofluorescence Assay (IFA)

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.