Test Code ESTRA Estradiol
Additional Codes
Epic: LAB523
Specimen Required
Collection Container/Tube: Serum SST
Submission Container/Tube: Plastic vial
Specimen Volume: 3.0 mL
Collection Instructions: Centrifuge and separate
cells after clot formation and within 4 hours of collection.
Separate serum from gel within 24 hours.
Specimen Minimum Volume
0.5 mL
Specimen Stability
Room temperature: 24 hours
Refrigerated on gel: 24 hours
Refrigerated off gel: 7 days
Frozen: 2 weeks
Reference Range
Normal
Menstruating Females:
Follicular Phase 21-251 pg/mL
Mid-Cycle Phase: 38-649 pg/mL
Luteal Phase 21-312 pg/mL
Postmenopausal Females not on HRT: <10-28 pg/mL
Postmenopausal Females on HRT: <10-144 pg/mL
Males: 11-44 pg/mL
Rejection Due To
Serum stored on gel >24 hours, unlabeled, mislabeled, wrong tube type, QNS, hemolysis index >500, gross lipemia (trigs >1000 mg/dl).
Report Available
Same day
Clinical Significance
Estradiol is the most potent natural estrogen in humans. It regulates reproductive function in females, and, with progesterone, maintains pregnancy. Most estradiol is secreted by the ovaries (non-pregnant women), although the testes (in men) and adrenal cortex (in men and women) secrete small amounts. During pregnancy, the placenta produces most of the circulating estradiol. Normal estradiol levels are lowest at menses and into the early follicular phase (25-75 pg/mL) and then rise in the late follicular phase to a peak of 200-600 pg/mL just before the LH surge, which is normally followed immediately by ovulation. As LH peaks, estradiol begins to decrease before rising again during the luteal phase (100-300 pg/mL). If conception does not take place, estradiol falls further to its lowest levels, and menses begins shortly thereafter. If conception occurs, estradiol levels continue to rise, reaching levels of 1,000-5,000 pg/mL during the first trimester, 5,000-15,000 pg/mL during second trimester, and 10,000-40,000 pg/mL during third trimester. At menopause, estradiol levels remain low. Because the ovaries produce most estradiol in normal women, estimation of this hormone is sometimes a gauge of ovarian function. In addition, monitoring estradiol levels is important in evaluating amenorrhea, precocious puberty, the onset of menopause, and infertility in men and women. Monitoring estradiol levels is essential during in vitro fertilization, because the timing of recovery of oocytes depends on follicular development, which in turn depends on the estradiol level.
Method Name
Chemiluminescent Micro-particle Immunoassay (CMIA)
Limitations
If the estradiol results are inconsistent with clinical
evidence, additional testing is suggested to confirm the
result.
Heterophilic antibodies in human serum can react with reagent
immunoglobulins, interfering with in vitro immunoassays.
LOINC
2243-4
Specimen Type
Serum
Day(s) Performed
Daily