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Test Code EVBPC Epstein-Barr Virus DNA, Qualitative Real-Time PCR

Specimen Required

1 mL whole blood or 1 mL serum or 1 mL CSF

Specimen Minimum Volume

0.3 mL

Specimen Stability

Whole blood
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable

All other specimens
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: 30 days

Rejection Due To

Hemolyzed whole blood • Heparin whole blood

Day(s) and Time(s) Performed

Set up: Daily; Report available: 1 day

Method Name

Real-Time Polymerase Chain Reaction

Collection Container / Tube

Preferred: EDTA (lavender-top
Other Acceptable Container: Potassium PPT EDTA (white-top), or ACD solution A or B (yellow-top) tube 1 mL CSF bronchoalveolar lavage, or urine collected in a leak-proof container 1 mL serum

Transport / Storage Conditions

Refrigerated (cold packs)
Tissue: Frozen

Special Instructions

CSF: Collect in a sterile, plastic container and store refrigerated or frozen.

Tissue: Collect in a sterile, plastic container and transport frozen.

Avoid repeated freezing and thawing of specimens. Do not freeze whole blood.

Reference Values

Not detected

CPT Codes

87798

Clinical Reference

Infection with EBV is common and is generally subclinical or presents as a self-limited illness. Reactivation of latent EBV in an immunocompromised person can lead to more serious results, including lymphoproliferative disorders or neurological disease. PCR methods may be useful in identifying EBV in a variety of clinical specimens.

Analytic Time

Set up: Daily; Report available: 1 day