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Test Code F8G Factor VIII Antigen

Specimen Required

1 mL frozen platelet-poor plasma

Specimen Minimum Volume

0.5 mL

Specimen Stability

Plasma
Room temperature: 4 hours
Refrigerated: 48 hours
Frozen (-20C): 30 days Frozen (-70C): 6 months

Day(s) and Time(s) Performed

As needed: at least 1x/month

Method Name

Immunoassay (IA)

Collection Container / Tube

Preferred: 3.2 % sodium citrate (light blue top)
Other Acceptable Container: None

Transport / Storage Conditions

Frozen

Special Instructions

Platelet poor plasma: Centrifuge light blue-top tube for 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic screw-cap vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic screw-cap vial. Plasma must be free of platelets (<10, 000/mcL). Freeze immediately and ship on dry ice. Do not thaw.

Reference Values

0.60-1.95 IU/mL

CPT Codes

85244

Analytic Time

2-31 days

Alias/See Also

LAB9290
Factor 8 antigen