Test Code FISHI FISH, AML, CBFB/MYH11, Inversion 16
Specimen Required
5 mL whole blood or 3 mL bone marrow
Specimen Minimum Volume
1 mL
Specimen Stability
Room temperature: See instructions Refrigerated: See instructins Frozen: See instructions
Rejection Due To
Received frozen
Method Name
Fluorescence in situ Hybridization (FISH)
Collection Container / Tube
Preferred: Sodium Heparin (green top) tube
Other Acceptable Container: Sodium heparin (royal blue-top) tube,
or sodium heparin lead-free (tan-top) tube
Transport / Storage Conditions
Room temperature
Special Instructions
Clinical history and reason for referral are required with test
order. Prior therapy/transplant information should be submitted
with test order.
Specimen requirements: 3-5 mL whole blood or 1-3
mL bone marrow collected in a sodium heparin tube.
Specimen viability decreases during transit. Send specimen
to testing laboratory for viability determination. Do not freeze.
Do not reject.
Note: If results are not possible, the test order may be canceled
and replaced by a "Cytogenetics Communication".
Reference Values
See Laboratory Report
Day(s) Performed
Set up: Daily; Report available: 5 days
CPT Codes
88271 (x2), 88275
Clinical Reference
This test is performed to detect the CBFB rearrangements such as inversion (16) and translocation t(16;16), by FISH (fluorescence in situ hybridization). This assay is useful for diagnosis of acute myeloid leukemia (AML) with inv(16) or t(16;16); CBFB-MYH11.