Test Code FISHT FISH, ALLTEL/AML1, Translocation 12,21
Specimen Required
5 mL whole blood collected in a sodium heparin (green-top) tube, 3 mL bone marrow collected in a sodium heparin (green-top) tube
Specimen Minimum Volume
1 mL
Specimen Stability
Room temperature: See instructions Refrigerated: See instructins Frozen: See instructions
Method Name
Fluorescence in situ Hybridization (FISH)
Includes
If results are not possible from the submitted specimen, the test order will be canceled and replaced with a Cytogenetics Communication.
Collection Container / Tube
Preferred: sodium heparin (green top) tube
Other Acceptable Container: Sodium heparin (royal blue-top) tube,
or sodium heparin lead-free (tan-top) tube
Transport / Storage Conditions
Room temperature
Special Instructions
Clinical history and reason for referral are required with test
order. Prior therapy/transplant information should be submitted
with test order.
Specimen Requirements: Bone marrow 1-3 mL, or
whole blood 3-5 mL collected in a sodium heparin tube.
Specimen viability decreases during transit. Send specimen to
testing laboratory for viability determination. Do not
freeze. Do not reject.
Reference Values
See Laboratory Report
Day(s) Performed
Set up: Daily; Report available: 5 days
CPT Codes
88271 (x2), 88275
Clinical Reference
This FISH (fluorescence in situ hybridization) procedure is used to detect the TEL/AML1 gene fusion resulting from a cytogenetically undetectable t(12;21)(p13;q22) translocation which is common in pediatric pre B-ALL. This test is useful for the evaluation of suboptimal specimens, cases with a normal karyotype, or when an abnormal karyotype lacking a specific/prognostically defined abnormality or demonstrating deletion 12p is found.