Test Code FLDH Lactate Dehydrogenase (LDH), Body Fluid
Additional Codes
Epic: LAB188
Specimen Required
Specimen Type: Body Fluid
Preferred Source: Synovial, Pleural, Ascites, Pericardial, Peritoneal, Amniotic
Collection Container/Type
Preferred: Green top tube (Lithium or Sodium heparin)
Acceptable: Sterile container
Specimen Volume: 3 mL
Collection Instructions:
- Specimen source indicated on specimen is required.
Specimen Minimum Volume
0.5 mL
Specimen Stability
Room Temperature: Unacceptable
Refrigerated : 5 days
Frozen: Not defined
Reference Range
See laboratory report
Rejection Due To
Unlabeled, mislabeled, wrong tube type, QNS, wrong preservative, exceeds specimen stability requirements.
Report Available
Same day
Clinical Significance
LD, an enzyme found in most major tissues, is released with cell damage caused by hypoxia, necrosis, or trauma. LD is elevated by a number pathologic conditions, most notably cardiac and hepatitis disease. Five electrophoretic isoenzymes are observed, with the relative ratios varying with the tissue source.
Method Name
Lactate to pyruvate (NADH)
Limitations
Do not use hemolyzed samples. Red cells contain 150 times
more LDH activity than serum.
Bilirubin has <2% effect at 60 mg/dl. LDH values vary
greatly with methodology.
Lipemia has < 10% interference at 2000 mg/dL triglycerides.
Specimens not centrifuged and/or exposed to heat will show falsely
elevated LD results.
Day(s) Performed
Daily