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Test Code FRFIT Rabies Antibody Endpoint

Reporting Name

Rabies RFFIT Endpoint, S

Performing Laboratory

RFFIT/K-State Rabies Laboratory Manhattan/K-State Innovation Center

Specimen Type

Serum


Specimen Required


Specimen Type: Serum

Container/Tube: Red

Specimen Volume: 2 mL

Collection Instructions: Draw blood in a plain red-top tube(s), Spin down and send 2 mL of serum refrigerated in a plastic vial.

 

Note:  1. Serum gel tube is acceptable, but must be poured off into plastic vial.

          2. Collection date is required.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  60 days

Reference Values

Reportable range is 0.1 to 15.0 IU/mL

Less than 0.1 IU/mL: Below detection limit

 

In humans a results of 0.5 IU/mL or higher is considered an acceptable response to rabies vaccination according to the World Health Organization (WHO) guidelines; see WHO and Advisory Committee on Immunization Practices documents for additional guidance.

 

Day(s) and Time(s) Performed

Monday, Wednesday, Thursday

CPT Code Information

86382

LOINC Code Information

Test ID Test Order Name Order LOINC Value
FRFIT Rabies RFFIT Endpoint, S 6524-3

 

Result ID Test Result Name Result LOINC Value
Z2071 Rabies Titer-Response 6524-3

Method Description

In RFFIT, your serum (the non-cellular portion of your blood) is first diluted fivefold (1part serum in 4 parts diluent). Further (serial fivefold) dilutions are performed, each of which contain less and less of your serum. These serum dilutions are mixed with a standard amount of live rabies virus and incubated. Whatever RVNA is present will neutralize the virus. Next, some tissue culture cells are added and the serum/virus/cells are incubated together. Whatever rabies virus is left (i.e., that which has not been neutralized by the antibody in your serum), will infect the cells and this can be seen under the microscope through the use of specific staining. Calculation of the endpoint titer is made from the percent of virus infected cells observed on the slide.

Analytic Time

3 - 4 weeks

Reject Due To

Hemolysis Mild OK; Gross reject
Lipemia Mild OK; Gross reject
Icterus NA
Other NA

Method Name

Serum Neutralization Fluorescent Antibody

Secondary ID

90330