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Test Code GANGL Ganglioside Antibody Panel, Serum

Reporting Name

Ganglioside Ab Panel, S

Useful For

Supporting the diagnosis of an autoimmune neuropathy

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Specimen Volume: 1 mL


Specimen Minimum Volume

0.5 mL

Testing Algorithm

Screening tests are performed for IgG and IgM antibodies to GM1 and GD1b. If positive, the appropriate titer will be performed at an additional charge.

 

See Ganglioside Antibody Panel Algorithm in Special Instructions.

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 28 days
  Frozen  28 days
  Ambient  72 hours

Reference Values

Profile Information:

IGG_M: Negative

IGM_M: Negative

IGG_A: Negative

IGM_A: Negative

IGG_D: Negative

IGM_D: Negative

 

Reflex Information:

IGMTS: <1:2000

IMMTS: <1:4000

IGATS: <1:16000

IMATS: <1:8000

IGDTS: <1:2000

IMDTS: <1:2000

Day(s) and Time(s) Performed

Monday, Thursday; 12 p.m.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

83516 x 6

83520 x 6 (if applicable)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
GM1B Ganglioside Ab Panel, S 82455-7

 

Result ID Test Result Name Result LOINC Value
4414 IgG Asialo. GM1 63212-5
4416 IgG Disialo. GD1b 94868-7
4412 IgG Monos. GM1 63243-0
4415 IgM Asialo. GM1 63384-2
4417 IgM Disialo. GD1b 94870-3
4413 IgM Monos. GM1 63247-1

Clinical Reference

1. Martinez JM, Snyder MR, Ettore M, et al: Composite ganglioside autoantibodies and immune treatment response in MMN and MADSAM. Muscle Nerve 2018;57:1000-1005 doi: 10.1002/mus.26051 2. Taylor BV, Gross L, Windebank AJ: The sensitivity and specificity of anti-GM1 antibody testing. Neurology 1996;47:951-955

3. Kaida K, Ariga T, Yu RK: Antiganglioside antibodies and their pathophysiological effects on Guillain-Barre syndrome and related disorders-a review. Glycobiology 2009;19:676-692 doi: 10.1093/glycob/cwp027

Method Description

Antiganglioside antibodies in serum are detected by enzyme-linked immunosorbent assays (ELISA). Ganglioside antigens (GM1, Asialo GM1, and GD1b) adsorbed to wells of ELISA plates are incubated with patient's serum or controls. The plates are washed and alkaline phosphatase conjugated antihuman IgG or IgM antibodies (ie, secondary) are added in a second incubation. The wash step is repeated and enzyme substrate is added. Absorbance is measured and results are expressed as antibody titer, ie, the greatest dilution at which the absorbance of wells that contain patient serum is greater than 2.0 times the mean absorbance of normal sera tested simultaneously.(Taylor BV, Gross L, Windebank AJ: The sensitivity and specificity of anti-GM1 antibody testing. Neurology 1996 October;47:951-955)

Analytic Time

5 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Forms

If not ordering electronically, complete, print, and send a Neurology Specialty Testing Client Test Request (T732) with the specimen.

Profile Information

Test ID Reporting Name Available Separately Always Performed
IGG_M IgG Monos. GM1 No Yes
IGM_M IgM Monos. GM1 No Yes
IGG_A IgG Asialo. GM1 No Yes
IGM_A IgM Asialo. GM1 No Yes
IGG_D IgG Disialo. GD1b No Yes
IGM_D IgM Disialo. GD1b No Yes

Secondary ID

83189

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IGMTS IgG Monos GM1 Titer, S No No
IMMTS IgM Monos GM1 Titer, S No No
IGATS IgG Asialo GM1 Titer, S No No
IMATS IgM Asialo GM1 Titer, S No No
IGDTS IgG Disialo GD1b Titer, S No No
IMDTS IgM Disialo GD1b Titer, S No No