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Test Code GBM Glomerular Basement Membrane Antibodies, IgG, Serum

Reporting Name

Glomerular Basement Membrane IgG Ab

Useful For

Evaluating patients with rapid onset renal failure or pulmonary hemorrhage, as an aid in the diagnosis of Goodpasture syndrome

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Advisory Information


If patient is being evaluated for autoimmune skin disease, order CIFS / Cutaneous Immunofluorescence Antibodies (IgG), Serum for evaluation of anti-intercellular substance (ICS) and antibasement membrane zone (BMZ) antibodies.



Specimen Required


Container/Tube: 

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL


Specimen Minimum Volume

0.35 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 21 days
  Frozen  21 days

Reference Values

<1.0 U (negative)                     

≥1.0 U (positive)

Reference values apply to all ages.

Day(s) and Time(s) Performed

Monday through Friday, 4 p.m.

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

83516

LOINC Code Information

Test ID Test Order Name Order LOINC Value
GBM Glomerular Basement Membrane IgG Ab 31254-6

 

Result ID Test Result Name Result LOINC Value
GBM Glomerular Basement Membrane IgG Ab 31254-6

Clinical Reference

1. Pusey CD: Anti-glomerular basement membrane disease. Kidney Int 2003;64:1535-1550

Method Description

Glomerular basement membrane (GBM) antigen is covalently coupled to polystyrene microspheres that are impregnated with fluorescent dyes to create a unique fluorescent signature. GBM antibodies, if present in diluted serum, bind to the GBM antigen on the microspheres. The microspheres are washed to remove extraneous serum proteins. Phycoerythrin (PE)-conjugated antihuman-IgG antibody is then added to detect IgG anti-GBM bound to the microspheres. The microspheres are washed to remove unbound conjugate, and bound conjugate is detected by laser photometry. A primary laser reveals the fluorescent signature of each microsphere to distinguish it from microspheres that are labeled with other antigens. A secondary laser reveals the level of PE fluorescence associated with each microsphere. Results are calculated by comparing the median fluorescence response for GBM microspheres to a 4-point calibration curve.(Package insert: Bio-Plex 2200 Vasculitis. Bio-Rad Laboratories, Hercules, CA 4/2012)

Analytic Time

Same day/1 day

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus OK

Method Name

Multiplex Flow Immunoassay

Forms

If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.

Secondary ID

8106