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Test Code GSTRN Gastrin, Serum

Reporting Name

Gastrin, S

Useful For

Investigation of patients with achlorhydria or pernicious anemia

 

Investigation of patients suspected of having Zollinger-Ellison syndrome

 

Diagnosis of gastrinoma

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Patient Preparation:

1. Fasting (8 hours) required

2. For 12 hours before specimen collection, do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.

3. If medically feasible, proton pump inhibitor (omeprazole, lansoprazole, dexlansoprazole, esomeprazole, pantoprazole, and rabeprazole) therapy should be discontinued 1 week before measurement of serum gastrin levels.

4. Drugs that interfere with gastrointestinal motility (eg, opioids) should be discontinued for at least 2 weeks before serum gastrin testing.

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. If multiple specimens are collected, submit each vial under a separate order.

2. Label specimens with corresponding collection time.

3. Centrifuge at refrigerated temperature within 2 hours of collection and immediately aliquot serum into plastic vial.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Frozen (preferred) 30 days
  Refrigerated  24 hours

Reference Values

<100 pg/mL

There is no evidence that fasting serum gastrin levels differ between adults and children. Although 8-hour fasts are difficult or impossible to enforce in small children, serum gastrin levels after shorter fasting periods (3-8 hours) may be 50% to 60% higher than the 8-hour fasting value.

 

For SI unit Reference Values, see https://www.mayocliniclabs.com/order-tests/si-unit-conversion.html

Day(s) and Time(s) Performed

Monday through Friday; 8 a.m.-1 p.m.

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

82941

LOINC Code Information

Test ID Test Order Name Order LOINC Value
GAST Gastrin, S 2333-3

 

Result ID Test Result Name Result LOINC Value
GAST Gastrin, S 2333-3

Clinical Reference

1. Ellison EC, Johnson JA: The Zollinger-Ellison syndrome: a comprehensive review of historical, scientific, and clinical considerations. Curr Probl Surg. 2009 Jan;46:13-106. doi: 10.1067/j.cpsurg.2008.09.001

2. McColl KE, Gillen D, El-Omar E: The role of gastrin in ulcer pathogenesis. Ballieres Best Pract Res Clin Gastroenterol. 2000 Feb;14:13-26. doi: 10.1053/bega.1999.0056

3. Dockray GJ, Varro A, Dimaline R, Wang T: The gastrins: their production and biological activities. Ann Rev Phys. 2001;63:119-139. doi: 10.1146/annurev.physiol.63.1.119

4. Brandi ML, Gagel R, Angeli A, et al: Consensus: guidelines for the diagnosis and therapy of MEN type 1 and type 2. J Clin Endocrinol Metab. 2001 Dec;86:5658-5671. doi: 10.1210/jcem.86.12.8070

5. Ward PC: Modern approaches to the investigation of vitamin B12 deficiency. Clin Lab Med. 2002 Jun;22:435-445. doi: 10.1016/s0272-2712(01)00003-8

6. Dacha S, Razvi M, Massaad J, Cai Q, Wehbi M: Hypergastrinemia. Gastroenterol Rep (Oxf). 2015 Aug;3(3):201-208. doi: 10.1093/gastro/gov004

7. Ahmed M, Ahmed S: Functional, diagnostic and therapeutic aspects of gastrointestinal hormones. Gastroenterology Res. 2019 Oct;12(5):233-244. doi: 10.14740/gr1219

Method Description

The Immulite 2000 Gastrin assay is a chemiluminescent, enzyme-labeled immunometric assay based on a ligand-labeled murine monoclonal capture antibody specific for gastrin and separation by antiligand-coated solid phase. The patient sample along with the ligand-labeled, antigastrin monoclonal antibody, an alkaline phosphatase-conjugated rabbit polyclonal antigastrin antibody, and an alkaline phosphatase-conjugated murine monoclonal antigastrin antibody are simultaneously incubated in the presence of the immobilized antiligand bead in a reaction tube. During the 60-minute incubation, gastrin molecules in the sample form antibody sandwich complexes that, in turn, bind to antiligand on the solid phase. Unbound conjugate is then removed by a centrifugal wash, after which luminogenic substrate is added and the reaction tube is incubated for an additional 5 minutes. The chemiluminescent substrate, a phosphate ester of adamantyl dioxetane, undergoes hydrolysis in the presence of alkaline phosphatase to yield an unstable intermediate. The continuous production of the intermediate results in the sustained emission of light. The bound complex and, thus, also the photon output, as measured by the luminometer is proportional to the concentration of gastrin in the sample.(Instruction manual: Immulite 2000 Gastrin. Siemens Medical Solutions Diagnostics; PIL2KGA-15)

Analytic Time

Same day/1 day

Reject Due To

Gross hemolysis Reject
Gross lipemia OK

Method Name

Automated Chemiluminescent Immunometric Assay

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-Oncology Test Request Form (T729)

-Benign Hematology Test Request Form (T755)