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Test Code HGBFP Hemoglobin, Free, Plasma

Additional Codes

Epic: LAB92

Quest: 514

Specimen Required

Specimen Type: Plasma, Heparin

Collection Container/Type

Preferred: Green top (Sodium heparin)

Acceptable: Green top (Lithium heparin)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: 

  1. Centrifuge plasma within 1 hour of collection.
  2. Transfer the plasma to a sterile, plastic, screw-cap vial.

Specimen Minimum Volume

0.2 mL

Specimen Stability

Room temperature: 7 days
Refrigerated: 7 days
Frozen: Unacceptable

Reference Range

<8.4 mg/dL

Day(s) and Time(s) Performed

Tues-Sat p.m.

Report Available

Next day

Clinical Significance

The presence of free hemoglobin in plasma is an indication of intravascular hemolysis resulting from numerousconditions, among which include Paroxysmal Nocturnal Hemoglobinuria (PNH), Sickle-Cell Disease (SCD), Thalassemias, Hereditary Spherocytosis, Microangiopathic Hemolytic Anemias, Pyruvate Kinase Deficiency, ABO Mismatch Transfusion Reaction, Cardiopulmonary Bypass or Mechanical Heart Valve-induced Anemia.
Reference: JAMA. 2005;293(13):1653-1662

Method Name

Colorimetry (C)

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

CPT Code(s)


Performing Laboratory

Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153