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HelpTest Code HGBFP Hemoglobin, Free, Plasma
Specimen Required
2 mL serum
Specimen Minimum Volume
0.2 mL
Specimen Stability
Room temperature: 7 days : Refrigerated: 7 days : Frozen: Unacceptable
Day(s) and Time(s) Performed
Set up: Tues-Sat p.m; Report available: Next day
Method Name
Colorimetry ©
Collection Container / Tube
Preferred: Lithium Heparin (green-top)
Other Acceptable Container: None
Transport Container
Plastic screw-cap vial
Transport / Storage Conditions
Room temperature
Special Instructions
Centrifuge plasma within 1 hour of collection. Transfer the plasma to a sterile, plastic, screw-cap vial.
Reference Values
<8.4 mg/dL
CPT Codes
83051
Clinical Reference
The presence of free hemoglobin in plasma is an indication of
intravascular hemolysis resulting from numerousconditions, among
which include Paroxysmal Nocturnal Hemoglobinuria (PNH), Sickle-Cell Disease (SCD), Thalassemias, Hereditary Spherocytosis,
Microangiopathic Hemolytic Anemias, Pyruvate Kinase Deficiency,
ABO Mismatch Transfusion Reaction,
Cardiopulmonary Bypass or Mechanical Heart Valve-induced
Anemia.
Reference: JAMA.
2005;293(13):1653-1662