Test Code HGBFP Hemoglobin, Free, Plasma
Additional Codes
Epic: LAB92
Quest: 514
Specimen Required
Specimen Type: Plasma, Heparin
Collection Container/Type
Preferred: Green top (Sodium heparin)
Acceptable: Green top (Lithium heparin)
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
- Centrifuge plasma within 1 hour of collection.
- Transfer the plasma to a sterile, plastic, screw-cap vial.
Specimen Minimum Volume
0.2 mL
Specimen Stability
Room temperature: 7 days
Refrigerated: 7 days
Frozen: Unacceptable
Reference Range
<8.4 mg/dL
Day(s) and Time(s) Performed
Tues-Sat p.m.
Report Available
Next day
Clinical Significance
The presence of free hemoglobin in plasma is an indication of
intravascular hemolysis resulting from numerousconditions, among
which include Paroxysmal Nocturnal Hemoglobinuria (PNH),
Sickle-Cell Disease (SCD), Thalassemias, Hereditary Spherocytosis,
Microangiopathic Hemolytic Anemias, Pyruvate Kinase Deficiency, ABO
Mismatch Transfusion Reaction, Cardiopulmonary Bypass or Mechanical
Heart Valve-induced Anemia.
Reference: JAMA. 2005;293(13):1653-1662
Method Name
Colorimetry (C)
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
CPT Code(s)
83051
Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153