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Test Code HIPAB Heparin-Induced Platelet Antibody

Specimen Required

1 mL random serum

Specimen Minimum Volume

0.5 mL

Specimen Stability

Room temperature: Unacceptable Refrigerated: Unacceptable Frozen: 6 months

Rejection Due To

Received room temperature • Gross hemolysis • Lipemia • Icteric • Serum separator tube (SST®)

Method Name

Immunoassay (IA)

Limitations

When heparin is stopped, antibody that may have been present disappears within weeks to months.

Collection Container / Tube

Preferred: Red Top Tube (no gel)
Other Acceptable Container: 3.2 % sodium citrate (light-blue)

Transport Container

Plastic screw-cap vial

Transport / Storage Conditions

Frozen

Special Instructions

Serum: Centrifuge red top tube 15 minutes at approximately 1500 g as soon as possible after clotting.
Plasma: Centrifuge blue top tube 20 minutes at approximately 2500 g. Remove plasma from the cells as soon as possible.

Reference Values

Heparin-Induced Platelet Antibody Negative
Patient O.D. ≤0.300 OD Units

Day(s) Performed

Set up: Daily; Report available: 1 day

CPT Codes

86022

Clinical Reference

Heparin can induce thrombocytopenia, a decreased platelet count, due to antibody stimulation. False positive results may be due to immune complexes. False negative results may be due to low titer, low avidity antibodies.