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Test Code IGDS Immunoglobulin D (IgD), Serum

Reporting Name

Immunoglobulin D (IgD), S

Useful For

Providing important information on the humoral immune status.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Advisory Information


MPSS / Monoclonal Protein Studies, Serum should be performed to distinguish between polyclonal and monoclonal IgD.



Specimen Required


Patient Preparation: Fasting preferred but not required

Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Specimen Volume: 1 mL


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 28 days
  Frozen  28 days
  Ambient  21 days

Reference Values

≤10 mg/dL

Day(s) and Time(s) Performed

Monday through Friday; Continuously until 3 p.m.

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

82784

LOINC Code Information

Test ID Test Order Name Order LOINC Value
IGD Immunoglobulin D (IgD), S 2460-4

 

Result ID Test Result Name Result LOINC Value
IGD Immunoglobulin D (IgD), S 2460-4

Clinical Reference

1. Blade J, Kyle RA: Immunoglobulin D multiple myeloma: Presenting features, response to therapy, and survival in a series of 53 cases. J Clin Oncol 1994;12(11):2398-2404

2. Kyle RA, Katzmann JA: Immunochemical characterization of immunoglobulins. In Manual of Clinical Laboratory Immunology. Fifth edition. Edited by NR Rose, E Conway de Macario, JD Folds, et al: Washington, DC, ASM Press,1997, pp 156-176

3. Tietz NW: In Clinical Guide to Laboratory Tests. Second edition. Philadelphia, WB Saunders Company, 1990, p 325

Method Description

In this Siemens Nephelometer II method, the light scattered onto the antigen-antibody complexes is measured. The intensity of the measured scattered light is proportional to the amount of antigen-antibody complexes in the sample under certain conditions. If the antibody volume is kept constant, the signal behaves proportionally to the antigen volume.

 

A reference curve is generated by a standard with a known antigen content on which the scattered light signals of the samples can be evaluated and calculated as an antigen concentration. Antigen-antibody complexes are formed when a sample containing antigen and the corresponding antiserum are put into a cuvette. A light beam is generated with an LED, which is transmitted through the cuvette. The light is scattered onto the immuno-complexes that are present. Antigen and antibody are mixed in the initial measurement, but no complex is formed yet. An antigen-antibody complex is formed in the final measurement.

 

The result is calculated by subtracting value of the final measurement from the initial measurement. The distribution of intensity of the scattered light depends on the ratio of the particle size of the antigen-antibody complexes to the radiated wavelength.(Unpublished Mayo method; Instruction manual: Siemens Nephelometer II, Version 3 Siemens, Inc., Newark, DE, 2008)

Analytic Time

Same day/1 day

Reject Due To

Gross hemolysis OK
Gross lipemia Reject
Gross icterus OK

Method Name

Nephelometry

Secondary ID

9272