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Test Code KLLCF Kappa/Lambda Light Chains, Free with Ratio and Reflex to Immunofixation

Specimen Required

2 mL serum

Specimen Minimum Volume

1 mL

Specimen Stability

Room temperature: 72 hours Refrigerated: 7 days Frozen: 30 days

Rejection Due To

Gross hemolysis • Grossly lipemic • Microbially contaminated serum • Specimens containing heavy visible particulate

Day(s) and Time(s) Performed

Set up: Mon-Fri; Report available: Next day

Method Name



If Kappa/Lambda, Free is <0.26 or >1.65, Immunofixation will be performed at an additional charge (CPT code(s): 86334).

Collection Container / Tube

Preferred: Gold SST Tube
Other Acceptable Container: None

Transport Container

Plastic screw-cap vial

Transport / Storage Conditions

Room temperature

Reference Values

Kappa Light Chain, Free, Serum 3.3-19.4 mg/L
Lambda Light Chain, Free, Serum 5.7-26.3 mg/L
Kappa/Lambda Light Chains Free with Ratio, Serum 0.26-1.65

CPT Codes

83520 (x2)

Clinical Reference

Measurement of the amounts of the different light chain types aids in the diagnosis and monitoring of multiple myeloma, lymphocytic neoplasms, Waldenstrom’s macroglobulinemia, and connective tissue diseases, such as systemic lupus erythematosus.