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Test Code KLLCF Kappa/Lambda Light Chains, Free with Ratio Reflex to Immunofixation, Serum

Additional Codes

Epic: LAB3222

Quest: 15122

Testing Algorithm

If Kappa/Lambda, Free is <0.26 or >1.65, Immunofixation will be performed at an additional charge (CPT code(s): 86334).

Specimen Required

Specimen Type: Serum

Collection Container/Type

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 2 mL

Specimen Minimum Volume

1 mL

Specimen Stability

Room temperature: 72 hours

Refrigerated: 7 days

Frozen: 30 days

Reference Range

Kappa Light Chain, Free, Serum 3.3-19.4 mg/L
Lambda Light Chain, Free, Serum 5.7-26.3 mg/L
Kappa/Lambda Light Chains Free with Ratio, Serum 0.26-1.65


Rejection Due To

Gross hemolysis • Grossly lipemic • Microbially contaminated serum • Specimens containing heavy visible particulate

Day(s) and Time(s) Performed


Report Available

2-7 days

Clinical Significance

Measurement of the amounts of the different light chain types aids in the diagnosis and monitoring of multiple myeloma, lymphocytic neoplasms, Waldenstrom's macroglobulinemia, and connective tissue diseases, such as systemic lupus erythematosus.

Method Name


CPT Code(s)

83521 (x2)

Performing Laboratory

Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153


549: Immunofixation, Serum
11234: Kappa/Lambda Light Chains, Free with Ratio, Serum


Light Chains