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Test Code LADV Adenovirus, Molecular Detection, PCR, Varies

Reporting Name

Adenovirus PCR

Useful For

Aiding in the diagnosis of adenovirus infections

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Varies


Necessary Information


Specimen source is required.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Body fluid

Sources: Pleural, peritoneal, ascites, pericardial, or amniotic

Container/Tube: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.

 

Specimen Type: Respiratory

Sources: Bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate

Container/Tube: Sterile container

Specimen Volume: 1 mL

 

Specimen Type: Spinal fluid

Container/Tube: Sterile vial

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.

 

Specimen Type: Stool

Supplies: Stool Collection Kit, Random (T635)

Container/Tube: Sterile container

Specimen Volume: 1 g

 

Specimen Type: Swab

Supplies:

-M4-RT (T605)

-Bartels FlexTrans VTM-3 mL (T892)

-Jiangsu VTM-3 mL (T891)

Sources: Nasal, throat, respiratory, genital, or ocular

Container/Tube: Multimicrobe media (M4-RT) and Eswabs

Specimen Volume: Entire specimen

Collection Instructions: Place swab back into a multimicrobe media (M4-RT, M4, or M5).

 

Specimen Type: Tissue

Supplies:

-M4-RT (T605)

-Bartels FlexTrans VTM-3 mL (T892)

-Jiangsu VTM-3 mL (T891)

Container/Tube: Sterile container containing 1 mL to 2 mL of sterile saline or multimicrobe medium (M4-RT, M4, or M5)

Specimen Volume: Entire collection

Collection Instructions: Collect fresh tissue specimen.

 

Specimen Type: Urine

Container/Tube: Sterile container

Specimen Volume: 1 mL

Collection Instructions: Collect a random urine specimen.


Specimen Minimum Volume

Body Fluid, Respiratory Specimen, Spinal Fluid, or Urine: 0.3 mL
Stool: 0.5 g
Swab or Tissue: NA

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Negative

Day(s) and Time(s) Performed

Monday through Friday; 6 a.m.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LADV Adenovirus PCR 39528-5

 

Result ID Test Result Name Result LOINC Value
SRC65 Specimen Source 31208-2
89074 Adenovirus PCR 39528-5

Clinical Reference

1. Buckwalter SP, Teo R, Espy MJ, et al: Real-time qualitative PCR for 57 human adenovirus types from multiple specimen sources. J Clin Microbiol2011;50(3):766-771 doi:10.1128/jcm.05629-11

2. Ebner K, Pinsker W, Lion T: Comparative sequence analysis of the hexon gene in the entire spectrum of human adenovirus serotypes: phylogenetic, taxonomic, and clinical implications. J Virol 2005;79:12635-12642

3. Ebner K, Suda M, Watzinger F, Lion T: Molecular detection and quantitative analysis of the entire spectrum of human adenoviruses by a two-reaction real-time PCR assay. J Clin Microbiol 2005;43:3049-3053

4. Jothikumar N, Cromeans TL, Hill VR, et al: Quantitative real-time PCR assays for the detection of human adenoviruses and identification of serotypes 40 and 41. Appl Environ Microbiol 2005;71:3131-3136

5 . Robinson C, Echavarria M: Adenovirus. In Manual of Clinical Microbiology. Edited by PR Murray, EJ Baron, JH Jorgensen, et al: Ninth edition. Washington, DC, ASM Press, 2007, pp 1589-1600

6. Thavagnanam S, Christie SN, Doherty GM, et al: Respiratory viral infection in lower airways o asymptomatic children. Acta Paediatr Mar;99(3):394-398

7. Kaneko H, Maruko I, Iida T, et al: The possibility of human adenovirus detection in the conjunctiva in asymptomatic cases during a nosocomial infection. Cornea Jun 2008;27(5):527-530

Method Description

Respiratory, swab, stool, tissue, and urine samples were processed according to specimen source. Viral nucleic acid is extracted by the MagNA Pure automated instrument (Roche Applied Science). Primers and fluorescence resonance energy transfer (FRET) probes target a relatively conserved 185-base pair region of the adenovirus penton gene. The LightCycler instrument (Roche Applied Science) amplifies and monitors the development of target nucleic acid sequences after the annealing step during PCR cycling. This automated PCR system rapidly detects amplicon development through stringent air-controlled temperature cycling in capillary cuvettes. The detection of amplified products is based on the FRET principle. For FRET product detection, a hybridization probe with a donor fluorophore, fluorescein, on the 3'-end is excited by an external light source and emits light that is absorbed by a second hybridization probe with an acceptor fluorophore, LC-Red 640, at the 5'-end. The acceptor fluorophore then emits a light of a different wavelength that can be measured with a signal that is proportional to the amount of specific PCR product.(Unpublished Mayo method)

Analytic Time

Monday through Thursday: 2 days; Friday, Saturday: 3 days

Reject Due To

Other Calcium alginate-tipped swab Wood swab Transport swab containing gel

Method Name

Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.