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Test Code LASF1 Lactic Acid, Spinal Fluid

Reporting Name

Lactic Acid, CSF

Useful For

Aid in differentiating between bacterial and viral meningitis

 

Aid in identifying increased anaerobic glycolysis or hypoxia associated with bacterial meningitis, cerebral infarction, cerebral arteriosclerosis, intracranial hemorrhage, hydrocephalus, traumatic brain injury, cerebral edema, epilepsy, and inborn errors of metabolism

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

CSF


Specimen Required


Specimen Type: Spinal fluid

Container/Tube: Sterile container

Specimen Volume: 1 mL

Collection Instructions: Centrifuge to remove any cellular material.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
CSF Frozen (preferred) 60 days
  Refrigerated  24 hours
  Ambient  3 hours

Reference Values

0-2 days: 1.1-6.7 mmol/L

3-10 days: 1.1-4.4 mmol/L

11 days-17 years: 1.1-2.8 mmol/L

>17 years: 1.1-2.4 mmol/L

Day(s) and Time(s) Performed

Monday through Sunday; Continuously

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

83605

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LASF1 Lactic Acid, CSF 2520-5

 

Result ID Test Result Name Result LOINC Value
LASF1 Lactic Acid, CSF 2520-5

Clinical Reference

1. Sakushima K, Hayashino Y, Kawaguchi T, et al: Diagnostic accuracy of cerebrospinal fluid lactate for differentiating bacterial meningitis from aseptic meningitis: A meta-analysis. J Infect 2011;62:255-262

2. Zhang W, Natowicz MR: Cerebrospinal fluid lactate and pyruvate concentrations and their ratio. Clin Biochem 2013;46:694-697

Method Description

Lactate concentration is determined using an enzymatic colorimetric method. L-lactate is oxidized to pyruvate by the specific enzyme lactate oxidase. Peroxidase is used to generate a colored dye using the hydrogen peroxide generated in the first reaction. The intensity of the color formed is directly proportional to the L-lactate concentration. It is determined by measuring the increase in absorbance.(Package insert: Roche Diagnostics Cobas 6000; LACT2 reagent package insert; Indianapolis, IN 46256. 02/2016)

Analytic Time

Same day/1 day

Reject Due To

Gross hemolysis OK
Gross lipemia OK

Method Name

Colorimetric

Secondary ID

601821