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Test Code LCJC JC Virus, Molecular Detection, PCR, Spinal Fluid

Reporting Name

JC Virus PCR, CSF

Useful For

Aids in diagnosing progressive multifocal leukoencephalopathy due to JC virus (JCV)

 

This test is not to be used as a diagnostic tool for Creutzfeldt-Jakob disease (CJD).

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

CSF


Specimen Required


Supplies: Aliquot Tube, 5 mL (T465)

Preferred: 12 x 75-mm screw cap vial (T465)

Acceptable: Sterile screw cap vial

Container/Tube: Sterile vial

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.


Specimen Minimum Volume

0.3 mL

Testing Algorithm

See Meningitis/Encephalitis Panel Algorithm in Special Instructions.

Specimen Stability Information

Specimen Type Temperature Time Special Container
CSF Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Negative

Day(s) Performed

Monday through Friday

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LCJC JC Virus PCR, CSF 33295-7

 

Result ID Test Result Name Result LOINC Value
88909 JC Virus PCR, CSF 33295-7

Clinical Reference

1. Safak M, Khalili K: An overview: human polyomavirus JC virus and its associated disorders. J Neurovirol 2006;9 Suppl 1:3-9

2. Khalili K, White MK: Human demyelinating disease and the polyomavirus JCV. Mult Scler 2006 Apr;12(2):133-142

3. Ahsan N, Shah KV: Polyomaviruses and human diseases. Adv Exp Med Biol 2006;577:1-18

4. Romero JR, Kimberlin DW: Molecular diagnosis of viral infections of the central nervous system. Clin Lab Med 2003 Dec;23(4):843-865

5. Chen Y, Bord E, Tompkins T, et al: Asymptomatic reactivation of JC virus in patients treated with natalizumab. N Engl J Med 2009 Sep 10;361(11):1067-1074

6. Egli A, Infanti L, Dumoulin A, et al: Prevalence of polyomavirus BK and JC infection and replication in 400 healthy donors. J Infect Dis 2009 Mar 15;199(6):837-846

Method Description

Viral nucleic acid is extracted from the specimen using the MagNA Pure automated instrument (Roche Applied Science). Primers are directed to the large T antigen gene, which is a conserved sequence specific for JC virus (JCV). This assay detects only JCV; it does not detect BK virus or Simian virus 40 (SV40) (other polyomaviruses). The LightCycler instrument (Roche Applied Science) amplifies and monitors the development of target nucleic acid sequences after the annealing step during PCR cycling. This automated PCR system can rapidly detect amplicon development through stringent air-controlled temperature cycling in capillary cuvettes. The detection of amplified products is based on the fluorescence resonance energy transfer (FRET) principle. For FRET product detection, a hybridization probe with a donor fluorophore, fluorescein, on the 3'-end is excited by an external light source and emits light that is absorbed by a second hybridization probe with an acceptor fluorophore, LC-Red 640, at the 5'-end. The acceptor fluorophore then emits a light of a different wavelength that can be measured with a signal that is proportional to the amount of specific PCR product.(Unpublished Mayo method)

Report Available

2 to 5 days

Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Method Name

Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Secondary ID

88909