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Test Code LDH Lactate Dehydrogenase (LDH)

Additional Codes

Epic: LAB96

Specimen Required

Specimen Type: Serum

Collection Container/Type

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 3.5 mL

Collection Instructions: Centrifuge and separate cells after clot formation and within 4 hours of collection. 

Additional Information: Hemolyzed specimens CANNOT be used because erythrocytes contain 150 times more LD activity.

Specimen Minimum Volume

0.10 mL

Specimen Stability

Room temperature: 7 days

Refrigerated: 4 days

Frozen: 6 weeks

Clinical Significance

    LDH is an enzyme found in the cells of many body tissues, including the heart, liver, kidneys, skeletal muscles, brain, RBC's, and lungs. It is responsible for converting muscle lactate into pyruvate, an essential step in producing cellular energy. It is composed of four peptide chains of 2 subunits (M form and H form) which results in up to 5 different isoenzymes which can be separated and quantitated by electrophoresis. Measurement of the total LDH activity in serum or plasma is non-specific and cannot differentiate the tissues of origin of the component isoenzymes.

LDH is used in the differential diagnosis of hemolytic anemia and as a tumor marker in some malignancies such as germ cell tumors.

LDH is elevated in hepatitis, glomerular nephritis, pulmonary embolism, muscle disease and many leukemias and lymphomas.

As LDH is a non-specific marker, it is used in combination with other markers in diagnosis and patient management.

Reference Range

0 - 1 month: 178 - 629 U/L

1 - 4 months: 158 - 373 U/L

4 - 7 months: 135 - 376 U/L

7 months - 1 year: 129 - 367 U/L

1 - 4 years: 164 - 286 U/L

4 - 7 years: 155 - 280 U/L

7 - 10 years: 141 - 237 U/L

10 - 13 years: 141 - 231 U/L

13 - 150 years: 125 - 331 U/L

Rejection Due To

Unlabeled, mislabeled, wrong tube type, QNS, hemolyzed.

Day(s) and Time(s) Performed

Daily

Report Available

Same day / 1 day

Method Name

IFCC Recommended forward reaction – Lactate to Pyruvate.

LOINC

14804-9