Test Code LLEP Leukemia/Lymphoma Evaluation
Specimen Required
5 mL peripheral blood
4 mL Bone Marrow
Specimen Minimum Volume
3 mL peripheral 1 mL bone marrow
Specimen Stability
Peripheral blood and bone marrow: Room temperature: 72 hours
(may extend to 5 days, depending on cell viability) Refrigerated:
Not established Frozen: Not established
Tissue and body fluids Room temperature: Not established
Refrigerated: 72 hours
Frozen: Unacceptable
Rejection Due To
See instructions
Day(s) and Time(s) Performed
Mon-Sat a.m.Method Name
Flow Cytometry (FC)
Includes
Initial markers evaluated: CD2, CD3, CD4, CD5, CD7, CD8, CD10,
CD11c, CD13, CD19, CD20, CD23, CD33, CD34, CD38, CD56, CD64, CD117,
HLA-DR, sKappa, sLambda. CD45 is used for
gating.
Additional markers may be performed based on the pathologist
review. These markers will be performed at an additional
charge (CPT code(s): 88185 for each
additional marker).
Collection Container / Tube
Peripheral Blood or Bone Marrow:
Preferred: Sodium Heparin (Green Top)
Other Acceptable Container: ACD-A
(yellow-top) or EDTA (lavendar-top) OR
bone marrow collected in a Pediatric Heparin Tube
Tissue or body fluids:
Any type is Other Acceptable Container
Transport / Storage Conditions
Peripheral blood and bone marrow: Room
temperature
Tissue and body fluids: Refrigerate (cold
packs)
Special Instructions
Peripheral blood and bone marrow: The tube must
be kept at room temperature and shipped to the lab immediately.
Tissue: Any tissue type is acceptable. Tissue size
is dependent upon leukocyte cellularity. (The tissue is
disaggregated into single cells so that a minimum of 50,000 cells
of interest are harvested.) Ship tissue in a sterile plastic
container with RPMI 1640 enriched with
FBS (10% FBS
RPMI). Absolutely no fixative should be
added. Refrigerate and ship immediately.
Body Fluids: Any body fluid is acceptable. Sample
size is dependent upon cellularity of the sample. (A minimum of
50,000 cells of interest in total volume of fluid). Place fluid in
sterile plastic container. Absolutely no fixative should be added.
Refrigerate and ship immediately.
A clinical indication and specimen source are required with each
specimen. If possible, submit CBC results
with differential or an EDTA tube of
peripheral blood.
Do not freeze and do not place in fixative.
Because of the critical nature of these specimens, the laboratory
will attempt to process all specimens received, regardless of age
of specimen.
Reference Values
See Laboratory Report
CPT Codes
88184, 88185 (x21), 88189
This test is not available for New York patient testing. For New
York patient testing use test code 37340.
Clinical Reference
To aid in the diagnosis of leukemia or lymphoma.
Initial markers evaluated: CD2, CD3, CD4, CD5, CD7, CD8, CD10,
CD11c, CD13, CD19, CD20, CD23, CD33, CD34, CD38, CD56, CD64, CD117,
HLA-DR, sKappa, sLambda. CD45 is used for
gating.
Analytic Time
2 Days