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Test Code LLEP Leukemia/Lymphoma Evaluation

Specimen Required

5 mL peripheral blood
4 mL Bone Marrow

Specimen Minimum Volume

3 mL peripheral 1 mL bone marrow

Specimen Stability

Peripheral blood and bone marrow: Room temperature: 72 hours (may extend to 5 days, depending on cell viability) Refrigerated: Not established Frozen: Not established
Tissue and body fluids Room temperature: Not established Refrigerated: 72 hours
Frozen: Unacceptable

Rejection Due To

See instructions

Day(s) and Time(s) Performed

Mon-Sat a.m.

Method Name

Flow Cytometry (FC)

Includes

Initial markers evaluated: CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD11c, CD13, CD19, CD20, CD23, CD33, CD34, CD38, CD56, CD64, CD117, HLA-DR, sKappa, sLambda. CD45 is used for gating.

Additional markers may be performed based on the pathologist review. These markers will be performed at an additional charge (CPT code(s): 88185 for each additional marker).

Collection Container / Tube

Peripheral Blood or Bone Marrow:
Preferred: Sodium Heparin (Green Top)
Other Acceptable Container: ACD-A (yellow-top) or EDTA (lavendar-top) OR bone marrow collected in a Pediatric Heparin Tube

Tissue or body fluids:
Any type is Other Acceptable Container

Transport / Storage Conditions

Peripheral blood and bone marrow: Room temperature
Tissue and body fluids: Refrigerate (cold packs)

Special Instructions

Peripheral blood and bone marrow: The tube must be kept at room temperature and shipped to the lab immediately.

Tissue: Any tissue type is acceptable. Tissue size is dependent upon leukocyte cellularity. (The tissue is disaggregated into single cells so that a minimum of 50,000 cells of interest are harvested.) Ship tissue in a sterile plastic container with RPMI 1640 enriched with FBS (10% FBS RPMI). Absolutely no fixative should be added. Refrigerate and ship immediately.

Body Fluids: Any body fluid is acceptable. Sample size is dependent upon cellularity of the sample. (A minimum of 50,000 cells of interest in total volume of fluid). Place fluid in sterile plastic container. Absolutely no fixative should be added. Refrigerate and ship immediately.

A clinical indication and specimen source are required with each specimen. If possible, submit CBC results with differential or an EDTA tube of peripheral blood.
Do not freeze and do not place in fixative.
Because of the critical nature of these specimens, the laboratory will attempt to process all specimens received, regardless of age of specimen.

Reference Values

See Laboratory Report

CPT Codes

88184, 88185 (x21), 88189
This test is not available for New York patient testing. For New York patient testing use test code 37340.

Clinical Reference

To aid in the diagnosis of leukemia or lymphoma.
Initial markers evaluated: CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD11c, CD13, CD19, CD20, CD23, CD33, CD34, CD38, CD56, CD64, CD117, HLA-DR, sKappa, sLambda. CD45 is used for gating.

Analytic Time

2 Days