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Test Code LNORO Norovirus PCR, Molecular Detection, Feces

Reporting Name

Norovirus PCR, F

Useful For

Diagnosis of gastrointestinal disease (diarrhea or vomiting) caused by norovirus genogroups 1 and 2


This test should not be used as a test-of-cure.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type


Shipping Instructions

Place vial in a sealed plastic bag and ship ambient. Specimens received at other temperature will be rejected.

Specimen Required

Supplies: C and S Vial (T058)

Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium containing phenol red as a pH indicator, either Cary-Blair, Para-Pak Culture and Sensitivity Media)

Specimen Volume: Representative portion of diarrheal fecal sample, 1 gram or 5 mL

Collection Instructions:

1. Collect fresh feces and place in preservative within 1 hour of collection.

2. Visibly formed feces are not consistent with Norovirus gastrointestinal disease and should not be submitted for testing.

Specimen Stability Information

Specimen Type Temperature Time Special Container
Fecal Ambient 7 days

Reference Values


Day(s) and Time(s) Performed

Monday through Friday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87798 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LNORO Norovirus PCR, F 88701-8


Result ID Test Result Name Result LOINC Value
65170 Norovirus G1 PCR 54905-5
47553 Norovirus G2 PCR 54906-3

Clinical Reference

1. National Center for Immunization and Respiratory Diseases, Division of Viral Diseases: Norovirus: Clinical Overview. Center for Disease Control and Prevention; 2013. Reviewed June 1, 2018. Accessed August 18, 2020. Available at

2. Echenique IA, Stosor V, Gallon L, et al: Prolonged norovirus infection after pancreas transplantation: a case report and review of chronic norovirus. Transpl Infect Dis. 2016 18(1):98-104

Method Description

This assay utilizes real-time, TaqMan-based polymerase chain reaction (PCR) technology to target the nonstructural polyprotein gene of norovirus G1 and G2. Nucleic acid is extracted from diarrheal stool samples in Cary-Blair media. Following extraction of viral RNA, reverse transcription is performed to convert norovirus genomic RNA to complementary DNA (cDNA). Real-time amplification and detection is then performed. Two separate real-time PCR reactions are performed for each sample, one specific for norovirus G1 and the second targeting norovirus G2. The assay is able to accurately detect and differentiate these genogroups in clinical stool samples.(Unpublished Mayo method)

Analytic Time

Monday through Thursday: 2 days; Friday, Saturday: 3 days

Reject Due To

Nonpreserved feces Transport media other than Cary-Blair or C and S Fecal swabs Visibly formed feces Frozen specimen Reject

Method Name

Real-Time Polymerase Chain Reaction (PCR)/RNA Probe Hybridization

Secondary ID