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Test Code NEZPP Zinc Protoporphyrin, Blood

Reporting Name

Zinc Protoporphyrin, B

Useful For

Evaluating iron deficiency


Monitoring treatment and environmental intervention of chronic lead poisoning

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Whole blood

Specimen Required

Patient Preparation: High concentrations of gadolinium and iodine are known to interfere with most metals tests. If either gadolinium- or iodine-containing contrast media has been administered, a specimen should not be collected for 96 hours.


-Metal Free B-D Tube (EDTA), 6 mL (T183)

-Metal Free (Lead only) EDTA Tube, 3 mL (T615)

-Microtainer (EDTA) Tube, 0.5 mL (T174)

-If ordering the EDTA trace element Vacutainer tube from BD, order catalog #368381.


Preferred: Royal blue-top BD Vacutainer Plus with EDTA blood collection tube (T183)

Acceptable: Tan-top (lead only) BD Vacutainer Plus with EDTA blood collection tube (T615) or BD Microtainer with EDTA (T174) or royal blue-top Monoject trace element blood collection tube

Specimen Volume: 1 mL

Collection Instructions:

1. See Trace Metals Analysis Specimen Collection and Transport in Special Instructions for complete instructions.

2. Send specimen in original tube.

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole blood Refrigerated 28 days

Reference Values

<70 mcmol ZPP/mol heme

Day(s) and Time(s) Performed

Monday through Friday; 5 p.m.

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
NEZPP Zinc Protoporphyrin, B 29763-0


Result ID Test Result Name Result LOINC Value
300009 Zinc Protoporphyrin, B 29763-0

Clinical Reference

1. Stanton NV, Gunter EW, Parsons PJ, et al: Empirically determined lead-poisoning screening cutoff for the Protofluor-Z hematofluorometer. Clin Chem 1989;35(10):2104-2107

2. Rosen JF: Preventing Lead Poisoning in Young Children. US Public Health Service, Centers for Disease Control, Atlanta, GA, 1991

3. Occupational Safety and Health Administration: OSHA Lead Standard-Requirements from the General Industry Standards Lead (1910, 1025), from 29 CFR 1910, 1025, A.M. Best Safety and Security-2000. Retrieved July 2016. Available at

4. Centers for Disease Control and Prevention. Screening Young Children for Lead Poisoning. Guidance for State and Local Public Health Officials. Atlanta, GA: US Dept of Health and Human Services. Public Health Service: November 1997. Available at

Method Description

Zinc protoporphyrin is measured on the Helena ProtoFluor-Z hematofluorometer using a multichannel front surface photofluorometer. When the sample is exposed to light, zinc protoporphyrin (ZPP) is excited and emits light. A second lens/filter system collects, filters, and focuses the light onto a photomultiplier tube (PMT). The PMT produces a current level in response to the light reaching it, which is proportional to the ZPP:heme ratio. During the reading, over 1,000 light-level readings are taken and averaged by the microcomputer and a value is displayed in mcmol ZPP/mol heme.(Instruction manual: Helena Laboratories ProtoFluor-Z Hematofluorometer 9/2013)

Analytic Time

1 day

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Method Name



1. Lead and Heavy Metals Reporting (T491) in Special Instructions

2. If not ordering electronically, complete, print, and send a Benign Hematology Test Request (T755) with the specimen.