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Test Code P19DQ Parvovirus B19 DNA, Qualitative Real-Time PCR [34296X]

Specimen Required

1 mL plasma collected in an EDTA (lavender-top), ACD solution B (yellow-top), or PPT potassium EDTA (white-top) tube, or
1 mL whole blood or bone marrow collected in EDTA (lavender-top), or ACD solution B (yellow-top) tube, or
1 mL amniotic fluid or amniotic fluid supernatant collected in a sterile leak-proof container, or
1 mL serum, or
Minimum: > 3.0 mm3 tissue collected in a sterile container

Specimen Minimum Volume

0.3 mL

Specimen Stability

Whole blood and bone marrow
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: Unacceptable

All other specimens
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days

Rejection Due To

Hemolyzed whole blood • Heparin plasma or whole blood

Day(s) and Time(s) Performed

Set up: Daily; Report available: 1 day

Method Name

Real-Time Polymerase Chain Reaction (PCR)

Collection Container / Tube

Other Acceptable Container:

Transport / Storage Conditions

Whole Blood, Bone Marrow, or Tissue: Refrigerated (cold packs)
All other specimens: Frozen

Reference Values

Not detected

CPT Codes

This test is not available for New York patient testing. For New York patient testing use test code 18577

Clinical Reference

Parvovirus B19 infection causes "Fifth Disease," a rash illness of childhood characterized by a bright red "slapped cheek" rash and a lacy rash on the trunk and limbs. Joint pain and swelling is more common in adults. Individuals with chronic hemolytic anemia such as sickle cell anemia may develop transient aplastic crisis with severe anemia. Immunocompromised individuals may develop chronic or recurrent bone marrow suppression. Infection during pregnancy can lead to fetal hydrops and spontaneous abortion. DNA testing in conjunction with B19 specific serology provides the most reliable evidence of a recent infection.

Analytic Time

Set up: Daily; Report available: 1 day