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Test Code PBNP NT-Pro B-Type Natriuretic Peptide, Serum

Additional Codes

Epic: LAB14240

Mayo: PBNP1

Specimen Required


Necessary Information


Patient's age and sex are required.



Specimen Required


Patient Preparation: For 12 hours before specimen collection do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Serum gel tubes should be centrifuged within 2 hours of collection.

2. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.


Secondary ID

615897

Useful For

Aids in the diagnosis of congestive heart failure using serum specimens

Method Name

Electrochemiluminescence Immunoassay

Reporting Name

NT-Pro BNP, S

Specimen Type

Serum

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Frozen (preferred) 365 days
  Refrigerated  7 days

Reject Due To

Gross hemolysis Reject

Reference Values

Males

0-2 day: 321-11,987 pg/mL

3-11 day: 263-5918 pg/mL

12 day-1 month: Not applicable

2 month-1 year: 37-646 pg/mL

2 years: 39-413 pg/mL

3 years-6 years: 23-289 pg/mL

7 years-14 years: ≤157 pg/mL

15 years-18 years: ≤158 pg/mL

19-39 years: <79 pg/mL

40-44 years: ≤72 pg/mL

45-54 years: ≤87 pg/mL

55-64 years: ≤88 pg/mL

≥65 years: ≤540 pg/mL

 

Females

0-2 day: 321-11,987 pg/mL

3-11 day: 263-5918 pg/mL

12 day-1 month: Not applicable

2 month-1 year: 37-646 pg/mL

2 years: 39-413 pg/mL

3 years-6 years: 23-289 pg/mL

7 years-14 years: < or=157 pg/mL

15 years-18 years: ≤158 pg/mL

19-39 years: <160 pg/mL

40-44 years: ≤162 pg/mL

45-54 years: ≤141 pg/mL

55-64 years: ≤226 pg/mL

≥65 years: ≤540 pg/mL

Clinical Reference

1. Januzzi JL, van Kimmenade RR, Lainchbury J, et al: NT-proBNP testing for diagnosis and short-term prognosis in acute destabilized heart failure: an international pooled analysis of 1256 patients: the international collaborative of NT-proBNP study. Eur Heart J. 2006 Feb;27(3):330-337. doi: 10.1093/eurheartj/ehi631

2. van Kimmenade RR, Pinto YM, Bayes-Genis A, et al: Usefulness of intermediate amino-terminal pro-brain natriuretic peptide concentrations for diagnosis and prognosis of acute heart failure. Am J Cardiol. 2006 Aug 1;98(3):386-390. doi: 10.1016/j.amjcard.2006.02.043

3. DeFilippi C, van Kimmenade R, Pinto YM: Amino-terminal pro-B-type natriuretic peptide testing in renal disease. Am J Cardiol. 2008 Feb 4;101(3A):82- 88. doi: 10.1016/j.amjcard.2007.11.029

4. Rifai N, Horwath AR, Wittwer CT, eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 6th ed. Elsevier; 2018

Method Description

This is an automated, double-incubation sandwich assay. In the first incubation, antigen from the patient specimen reacts with biotinylated monoclonal sheep N-terminal pro brain natriuretic peptide (NT-proBNP) antibody and monoclonal NT-proBNP antibody labeled with ruthenium complex. During the second incubation, streptavidin-labeled microparticles are added, and the resulting complex is bound to the solid-phase via biotin-streptavidin interaction. The resulting reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of an electrode. Unbound substances are washed away. Voltage is then applied to the electrode, which induces chemiluminescent emission that is measured by a photomultiplier. Results are obtained by comparing this measurement against the calibration curve. The Roche E Modular NT-proBNP assay standardization is traceable to an internal Roche standard. Assigned values for calibrators are traceable to this standardization.

 

The Roche NT-proBNP assay is automated and more precise than the Biosite BNP assay used previously. In addition, in vitro NT-proBNP is more stable than BNP.(Package insert: ProBNP II, Roche Diagnostics; V 12.0. 07/2021)

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 to 2 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

83880

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PBNP1 NT-Pro BNP, S 83107-3

 

Result ID Test Result Name Result LOINC Value
PBNP1 NT-Pro BNP, S 83107-3