Sign in →

Test Code PCAB Parietal Cell Antibodies, IgG, Serum

Reporting Name

Parietal Cell Ab, IgG, S

Useful For

Evaluating patients suspected of having pernicious anemia or immune-mediated deficiency of vitamin B12 with or without megaloblastic anemia

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL


Specimen Minimum Volume

0.45 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 21 days
  Frozen  21 days

Reference Values

Negative: ≤20.0 Units

Equivocal: 20.1-24.9 Units

Positive: ≥25.0 Units 

Reference values apply to all ages.

Day(s) and Time(s) Performed

Tuesday, Friday; 3 p.m.

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

83516

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PCAB Parietal Cell Ab, IgG, S 40960-7

 

Result ID Test Result Name Result LOINC Value
PCAB Parietal Cell Ab, IgG, S 40960-7

Clinical Reference

1. Toh BH, Van Driel IR, Gleeson PA: Pernicious anemia. N Eng J Med 1997;337(20):1441-1448

2. Lahner E, Annibale B: Pernicious anemia: new insights from a gastroenterological point of view. World J Gastroenterol 2009;15(41):5121-5128

3. Lahner E, Normal GL, Severi C, et al: Reassessment of intrinsic factor and parietal cell autoantibodies in atrophic gastritis with respect to cobalamin deficiency. Am J Gastroenterol 2009;104(8):2071-2079

4. Product Insert: QUANTA Lite GPA, INOVA Diagnostics, Inc., San Diego, CA

Method Description

Purified H(+)/K(+) ATPase antigen, isolated from pig gastric mucosa, is bound to the wells of a polystyrene microwell plate under conditions that will preserve the antigen in its native state. Prediluted controls and diluted patient sera are added to separate wells, allowing any H(+)/K(+) ATPase antibodies present to bind to the immobilized antigen. Unbound sample is washed away and an enzyme-labeled antihuman IgG conjugate is added to each well. A second incubation allows the enzyme-labeled antihuman IgG to bind to any patient antibodies, which have become attached to the microwells. After washing away any unbound enzyme-labeled antihuman IgG, the remaining enzyme activity is measured by adding a chromogenic substrate and measuring the intensity of the color that develops. The assay is evaluated spectrophotometrically by measuring and comparing the color intensity that develops in the patient wells with the color in the control wells.(Package insert: QUANTA Lite GPA, INOVA Diagnostics, Inc., December 2002, Revision 1)

Analytic Time

1 day

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus OK
Other Heat-treated specimen

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Secondary ID

83728