Test Code PEANT Peanut, IgE with Reflex to Peanut Components, IgE, Serum
Reporting Name
Peanut Component Reflex, SUseful For
Evaluation of patients with suspected peanut allergy
Evaluation of patients with possible peanut cross-reactivity
Performing Laboratory

Specimen Type
SerumOrdering Guidance
For a listing of allergens available for testing, see Allergens - Immunoglobulin E (IgE) Antibodies in Special Instructions
Specimen Required
Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Specimen Volume: 2 mL
Specimen Minimum Volume
0.75 mL
Testing Algorithm
Testing begins with analysis of peanut IgE. If peanut IgE is negative (<0.10 kU/L), testing is complete.
If peanut IgE is 0.10 kU/L or greater, then 7 peanut components (Ara h 2, Ara h 1, Ara h 3, Ara h 6, Ara h 8, Ara h 9, and profilin Bet v2) are performed at an additional charge.
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 14 days | |
Frozen | 90 days | ||
Ambient | 7 days |
Special Instructions
Reference Values
Class |
IgE kU/L |
Interpretation |
0 |
<0.10 |
Negative |
0/1 |
0.10-0.34 |
Borderline / Equivocal |
1 |
0.35-0.69 |
Equivocal |
2 |
0.70-3.49 |
Positive |
3 |
3.50-17.4 |
Positive |
4 |
17.5-49.9 |
Strongly positive |
5 |
50.0-99.9 |
Strongly positive |
6 |
≥100 |
Strongly positive |
Concentrations ≥0.70 kU/L (Class 2 and above) will flag as abnormally high.
Day(s) Performed
Monday through Friday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86003
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
PEANT | Peanut Component Reflex, S | 6206-7 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
PNUT | Peanut, IgE, S | 6206-7 |
Clinical Reference
1. Sicherer SH, Wood RA: Advances in diagnosing peanut allergy. J Allergy Clin Immunol Pract. 2013 Jan;1(1):1-13. doi: 10.1016/j.jaip.2012.10.004
2. Eller E, Bindslev-Jensen C: Clinical value of component-resolved diagnostics in peanut-allergic patients. Allergy. 2013 Feb;68(2):190-194. doi: 10.1111/all.12075
3. Hong X, Caruso D, Kumar R, et al: IgE, but not IgG4, antibodies to Ara h 2 distinguish peanut allergy from asymptomatic peanut sensitization. Allergy. 2012 Dec;67(12):1538-1546. doi: 10.1111/all.12047
4. Asarnoj A, Nilsson C, Lidholm J, et al: Peanut component Ara h 8 sensitization and tolerance to peanut. J Allergy Clin Immunol. 2012 Aug;130(2):468-472. doi: 10.1016/j.jaci.2012.05.019
5. Klemans RJ, van Os-Medendorp H, Blankestijn M, Bruijzeel-Koomen CA, Knol EF, Knulst AC: Diagnostic accuracy of specific IgE to components in diagnosing peanut allergy: a systematic review. Clin Exp Allergy. 2015 Apr;45(4):720-730. doi: 10.1111/cea.12412
6. Koid AE, Chapman MD, Hamilton RG, et al: Ara h 6 complements Ara h 2 as an important marker for IgE reactivity to peanut. J Agric Food Chem. 2014 Jan 8;62(1):206-213. doi:10.1021/jf4022509
7. Bublin M, Breiteneder H: Cross-reactivity of peanut allergens. Curr Allergy Asthma Rep. 2014 Apr;14(4):426. doi: 10.1007/s11882-014-0426-8
8. Chan ES, Greenhawt MJ, Fleischer DM, Caubet JC: Managing cross-reactivity in those with peanut allergy. J Allergy Clin Immunol Pract. 2019 Feb;7(2):381-386. doi: 10.1016/j.jaip.2018.11.012
9. Simberloff T, Parambi R, Bartnikas LM, el al: Implementation of a standardized clinical assessment and management plan (SCAMP) for food challenges. J Allergy Clin Immunol Pract. 2017 Mar-Apr;5(2):335-344. doi:10.1016/j.jaip.2016.05.021
Method Description
Specific IgE from the patient's serum reacts with the allergen of interest, which is covalently coupled to an ImmunoCAP. After washing away nonspecific IgE, enzyme-labeled anti-IgE antibody is added to form a complex. After incubation, unbound anti-IgE is washed away and the bound complex is then incubated with a developing agent. After stopping the reaction, the fluorescence of the eluate is measured. Fluorescence is proportional to the amount of specific IgE present in the patient's sample (ie, the higher the fluorescence value, the more IgE antibody is present).(Package insert: ImmunoCAP System Specific IgE FEIA. Phadia; Rev 06/2019)
Report Available
Same day/1 to 3 daysReject Due To
Gross hemolysis | OK |
Gross lipemia | OK |
Gross icterus | OK |
Method Name
Fluorescent Enzyme Immunoassay (FEIA)
Forms
If not ordering electronically, complete, print, and send an Allergen Test Request (T236) with the specimen.
Secondary ID
64756Reflex Tests
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
PNTCP | Peanut Components, IgE, S | No | No |