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Test Code RBABM Measles (Rubeola) Antibodies, IgM, Serum

Reporting Name

Measles (Rubeola) Ab, IgM, S

Useful For

Determining acute-phase infection with rubeola (measles) virus using IgM antibody testing

 

Aiding in the identification of nonimmune individuals through IgM antibody testing

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Container/Tube: 

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL


Specimen Minimum Volume

0.2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reference Values

Negative

Reference values apply to all ages.

Day(s) and Time(s) Performed

Monday through Saturday; 9 a.m.

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86765

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ROM Measles (Rubeola) Ab, IgM, S 35276-5

 

Result ID Test Result Name Result LOINC Value
80979 Measles (Rubeola) Ab, IgM, S 35276-5

Clinical Reference

1. Liebert UG: Measles virus infections of the central nervous system. Intervirology 1997;40:176-184

2. Norrby E, Kristensson K: Measles virus in the brain. Brain Res Bull 1997;44:213-220

3. Sable CA, Hayden FG: Orthomyxoviral and paramyxoviral infections in transplant patients. Infect Dis Clin North Am 1995;9:987-1003

4. Matsuzono Y, Narita M, Satake A, et al: Measles encephalomyelitis in a patient with a history of vaccination. Acta Paediatr Jpn 1995;37:374-376

5. Cremer, NE, Devlin VL, Riggs JL, Hagens SJ: 1984. Anomalous antibody responses in viral infection: specific stimulation or polyclonal activation? J Clin Microbio 1984;20:468-472

6. Gershon AA, Krugman S: Measles virus. In Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections. Fifth edition. Edited by EH Lennette, NJ Schmidt. Washington, DC, American Public Health Association, Inc., 1979;665-693

Method Description

The presence of IgM-class antibody to measles is determined by an indirect immunofluorescence assay (IFA). After removal of IgG by specific immunoglobulin antibody, the serum is incubated with measles antigen, which is adhered to a glass microscope slide. Antibodies, if present, will bind to the antigen forming stable antigen-antibody complexes. If no antibodies are present, the complexes will not be formed and the serum components will be washed away. Fluorescein-labeled antihuman-IgM antibody is added to the reaction side and binds to IgM antibodies, if present. This results in a positive reaction of bright apple-green fluorescence when viewed with a fluorescence microscope.(Package insert: Measles Virus Antigen Substrate Slide, BION Enterprises, Des Plaines, IL, 4/2012)

Analytic Time

Same day/1 day

Reject Due To

Gross hemolysis Gross reject
Gross lipemia Gross reject
Other Heat-inactivated specimen

Method Name

Immunofluorescence Assay (IFA)

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.