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HelpTest Code RBABM Measles (Rubeola) Antibodies, IgM, Serum
Reporting Name
Measles (Rubeola) Ab, IgM, SUseful For
Determining acute-phase infection with rubeola (measles) virus using IgM antibody testing
Aiding in the identification of nonimmune individuals through IgM antibody testing
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumSpecimen Required
Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Specimen Volume: 0.5 mL
Specimen Minimum Volume
0.2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 14 days |
Reference Values
Negative
Reference values apply to all ages.
Day(s) and Time(s) Performed
Monday through Saturday; 9 a.m.
Test Classification
This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86765
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ROM | Measles (Rubeola) Ab, IgM, S | 35276-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 80979 | Measles (Rubeola) Ab, IgM, S | 35276-5 |
Clinical Reference
1. Liebert UG: Measles virus infections of the central nervous system. Intervirology 1997;40:176-184
2. Norrby E, Kristensson K: Measles virus in the brain. Brain Res Bull 1997;44:213-220
3. Sable CA, Hayden FG: Orthomyxoviral and paramyxoviral infections in transplant patients. Infect Dis Clin North Am 1995;9:987-1003
4. Matsuzono Y, Narita M, Satake A, et al: Measles encephalomyelitis in a patient with a history of vaccination. Acta Paediatr Jpn 1995;37:374-376
5. Cremer, NE, Devlin VL, Riggs JL, Hagens SJ: 1984. Anomalous antibody responses in viral infection: specific stimulation or polyclonal activation? J Clin Microbio 1984;20:468-472
6. Gershon AA, Krugman S: Measles virus. In Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections. Fifth edition. Edited by EH Lennette, NJ Schmidt. Washington, DC, American Public Health Association, Inc., 1979;665-693
Method Description
The presence of IgM-class antibody to measles is determined by an indirect immunofluorescence assay (IFA). After removal of IgG by specific immunoglobulin antibody, the serum is incubated with measles antigen, which is adhered to a glass microscope slide. Antibodies, if present, will bind to the antigen forming stable antigen-antibody complexes. If no antibodies are present, the complexes will not be formed and the serum components will be washed away. Fluorescein-labeled antihuman-IgM antibody is added to the reaction side and binds to IgM antibodies, if present. This results in a positive reaction of bright apple-green fluorescence when viewed with a fluorescence microscope.(Package insert: Measles Virus Antigen Substrate Slide, BION Enterprises, Des Plaines, IL, 4/2012)
Analytic Time
Same day/1 dayReject Due To
| Gross hemolysis | Gross reject |
| Gross lipemia | Gross reject |
| Other | Heat-inactivated specimen |
Method Name
Immunofluorescence Assay (IFA)
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.