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Test Code REPTA Reptilase Clotting Time

Additional Codes

Epic: LAB1136

Quest: 37700

Testing Algorithm

Testing Algorithm

Specimen Required

Specimen Type: Platelet-poor plasma

Collection Container/Type: Light-blue tube (citrate)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: 

  1. Draw blood into a light blue (sodium citrate) tube.  Be sure to fill tubes completely.
  2. Mix gently 5 to 6 times (do not over mix).
  3. Centrifuge the sample for 10 minutes.
  4. Using a pipette, aliquot plasma into a plastic vial being careful not disturb the buffy coat.
  5. Centrifuge the plasma sample a second time for 10 minutes
  6. Using a pipette, aliquot the top portion of plasma into a plastic vial.  Be careful not to disturb the sediment at the bottom (leave approx. 0.25 mL of plasma at the bottom).
  7. Label each tube as "Plasma."
  8. Immediately freeze samples.

Additional Information:

  • Please submit a separate, frozen vial for each special coagulation assay ordered.

Specimen Minimum Volume

0.5 mL

Specimen Stability

Room temperature: Unacceptable

Refrigerated: Unacceptable

Frozen -20°C: 30 days

Frozen -70°C: >30 days

Reference Range

14-20 seconds

Day(s) and Time(s) Performed


Report Available

Next day

Method Name

Clot Based Assay

CPT Code(s)


Performing Laboratory

Quest Diagnostics Nichols Institute
14225 Newbrook Dr
Chantilly, VA 20153