Test Code RIBOS Ribosome P Antibodies, IgG, Serum
Reporting Name
Ribosome P Ab, IgG, SUseful For
As an adjunct in the evaluation of patients with lupus erythematosus (LE)
Aids in the differential diagnosis of neuropsychiatric symptoms in patients with LE
Performing Laboratory

Specimen Type
SerumSpecimen Required
Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Specimen Volume: 0.5 mL
Specimen Minimum Volume
0.35 mL
Testing Algorithm
For more information see Connective Tissue Disease Cascade.
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 21 days | |
Frozen | 21 days |
Special Instructions
Reference Values
<1.0 U (negative)
≥1.0 U (positive)
Reference values apply to all ages.
Day(s) Performed
Monday through Saturday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
83516
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
RIB | Ribosome P Ab, IgG, S | 53892-6 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
RIB | Ribosome P Ab, IgG, S | 53892-6 |
Clinical Reference
1. Bonfa E, Golombek SJ, Kaufman LD, et al: Association between lupus psychosis and anti-ribosomal P protein antibodies. N Engl J Med 1987;317:265-271
2. Bonfa E, Elkon KB: Clinical and serologic associations of the anti-ribosomal P protein antibody. Arthritis Rheum 1986;29:981-985
Method Description
Affinity-purified ribosome P antigens are coupled covalently to polystyrene microspheres, which are impregnated with fluorescent dyes to create a unique fluorescent signature. Ribosome P antibodies, if present in diluted serum, bind to ribosome P antigen on the microspheres. The microspheres are washed to remove extraneous serum proteins. Phycoerythrin (PE)-conjugated, antihuman IgG antibody is then added to detect IgG anti-ribosome P antibodies bound to the microspheres. The microspheres are washed to remove unbound conjugate, and bound conjugate is detected by laser photometry. A primary laser reveals the fluorescent signature of each microsphere to distinguish it from microspheres that are labeled with other antigens, and a secondary laser reveals the level of PE fluorescence associated with each microsphere. Results are calculated by comparing the median fluorescence response for ribosome P microspheres to a 4-point calibration curve.(Package insert: Bioplex 2200 ANA Screen. Bio-Rad Laboratories, Hercules, CA 11/2011)
Report Available
Same day/1 to 3 daysReject Due To
Gross hemolysis | Reject |
Gross lipemia | Reject |
Gross icterus | OK |
Method Name
Multiplex Flow Immunoassay