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Test Code RPR2 Respiratory Profile, Region 2, Mid-Atlantic (DC, DE, MD, NC, VA), Serum

Reporting Name

Resp Profile, Reg 2, Mid-Atlantic

Useful For

Assessing sensitization to various inhalant allergens commonly found in the Mid-Atlantic region including the District of Columbia, Delaware, Maryland, North Carolina and Virginia

 

Defining the allergen responsible for eliciting signs and symptoms

 

Identifying allergens:

-Responsible for allergic disease and/or anaphylactic episode

-To confirm sensitization prior to beginning immunotherapy

-To investigate the specificity of allergic reactions to insect venom allergens, drugs, or chemical allergens

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Ordering Guidance


For a listing of allergens available for testing, see Allergens - Immunoglobulin E (IgE) Antibodies in Special Instructions



Specimen Required


Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Specimen Volume: 1.7 mL


Specimen Minimum Volume

1.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  90 days

Reference Values

Specific IgE:

Class

IgE kU/L

Interpretation

0

<0.35

Negative

1

0.35-0.69

Equivocal

2

0.70-3.49

Positive

3

3.50-17.4

Positive

4

17.5-49.9

Strongly positive

5

50.0-99.9

Strongly positive

6

≥100

Strongly positive

Reference values apply to all ages.

 

Total IgE:

Results Reported in kU/L

Age

Reference interval

0-5 months

≤13

6-11 months

≤34

1 and 2 years

≤97

3 years

≤199

4-6 years

≤307

7 and 8 years

≤403

9-12 years

≤696

13-15 years

≤629

16 and 17 years

≤537

18 years and older

≤214

Day(s) Performed

Monday through Friday

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

82785-IgE

86003 x 23-Each individual allergen

LOINC Code Information

Test ID Test Order Name Order LOINC Value
RPR2 Resp Profile, Reg 2, Mid-Atlantic 48825-4

 

Result ID Test Result Name Result LOINC Value
ALTN Alternaria Tenuis, IgE 6020-2
ASP Aspergillus Fumigatus, IgE 6025-1
BERG Bermuda Grass, IgE 6041-8
BIR Silver Birch, IgE 15283-5
BXMPL Box Eld/Maple, S, IgE 7155-5
CAT Cat Epithelium, IgE 6833-8
CED Mountain Cedar, IgE 6178-8
CLAD Cladosporium, IgE 53760-5
COCR Cockroach, IgE 6078-0
CTWD Cottonwood, IgE 6090-5
DF House Dust Mites/D.F., IgE 6095-4
DOGD Dog Dander, IgE 6098-8
DP House Dust Mites/D.P., IgE 6096-2
ELM Elm, IgE 6109-3
IGE Immunoglobulin E (IgE), S 19113-0
JOHN Johnson Grass, IgE 6152-3
MULB Mulberry, IgE 6281-0
OAK Oak, IgE 6189-5
PENL Penicillium, IgE 6212-5
RRRP Rough Pigweed, IgE 6233-1
SORR Red Sorrel, IgE 6244-8
SRW Short Ragweed, IgE 6085-5
TIMG Timothy Grass, IgE 6265-3
PCANH Pecan Hickory, IgE 6208-3

Clinical Reference

1. Homburger HA, Hamilton RG: Chapter 55: Allergic diseases. In Henry's Clinical Diagnosis and Management by Laboratory Methods. 23rd edition. Edited by RA McPherson, MR Pincus. Elsevier, 2017, pp 1057-1070

2. Bernstein IL, Li JT, Bernstein DI, et al: Allergy diagnostic testing: An updated practice parameter. Ann Allergy Asthma Immunol 2008 Mar;100(3 Suppl 3):S1-148

Method Description

Specific IgE:

Specific IgE from the patient's serum reacts with the allergen of interest, which is covalently coupled to an ImmunoCAP. After washing away nonspecific IgE, enzyme-labeled anti-IgE antibody is added to form a complex. After incubation, unbound anti-IgE is washed away and the bound complex is then incubated with a developing agent. After stopping the reaction, the fluorescence of the eluate is measured. Fluorescence is proportional to the amount of specific IgE present in the patient's sample (ie, the higher the fluorescence value, the more IgE antibody is present).(Package insert: ImmunoCAP System Specific IgE FEIA, Uppsala, Sweden Rev 06/2019)

 

Total IgE:

Anti-IgE, covalently coupled to ImmunoCAP, reacts with the IgE in a serum specimen. After washing, enzyme-labeled anti-IgE antibodies are added to form a complex. After incubation, unbound enzyme-labeled anti-IgE is washed away and the bound complex is incubated with a developing agent. After stopping the reaction, fluorescence of the eluate in the well is measured. The fluorescence is directly proportional to the concentration of IgE in the test specimen.(Package insert: Phadia CAP System IgE FEIA. issued August 2000, revised June 2010)

Report Available

1 to 3 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK

Method Name

Fluorescence Enzyme Immunoassay (FEIA)

Profile Information

Test ID Reporting Name Available Separately Always Performed
IGE Immunoglobulin E (IgE), S Yes Yes
DP House Dust Mites/D.P., IgE Yes Yes
DF House Dust Mites/D.F., IgE Yes Yes
CAT Cat Epithelium, IgE Yes Yes
DOGD Dog Dander, IgE Yes Yes
BERG Bermuda Grass, IgE Yes Yes
TIMG Timothy Grass, IgE Yes Yes
JOHN Johnson Grass, IgE Yes Yes
COCR Cockroach, IgE Yes Yes
PENL Penicillium, IgE Yes Yes
CLAD Cladosporium, IgE Yes Yes
ASP Aspergillus Fumigatus, IgE Yes Yes
ALTN Alternaria Tenuis, IgE Yes Yes
BXMPL Box Eld/Maple, S, IgE Yes Yes
BIR Silver Birch, IgE Yes Yes
CED Mountain Cedar, IgE Yes Yes
OAK Oak, IgE Yes Yes
ELM Elm, IgE Yes Yes
CTWD Cottonwood, IgE Yes Yes
PCANH Pecan Hickory, IgE Yes Yes
MULB Mulberry, IgE Yes Yes
SRW Short Ragweed, IgE Yes Yes
RRRP Rough Pigweed, IgE Yes Yes
SORR Red Sorrel, IgE Yes Yes