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Test Code SMUAB Smooth Muscle Antibody Screen, Serum

Specimen Required

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.8 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial


If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-General Request (T239)

-Gastroenterology and Hepatology Test Request (T728)

Secondary ID


Useful For

Evaluating patients with chronic liver disease in whom the diagnosis of chronic active autoimmune hepatitis is suspected

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
SMAT Smooth Muscle Ab Titer, S No No

Testing Algorithm

If smooth muscle antibody (SMA) screen is positive then the SMA titer will be performed at an additional charge.

Method Name

Indirect Immunofluorescence

Reporting Name

Smooth Muscle Ab Screen, S

Specimen Type


Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 21 days
  Frozen  21 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus OK

Reference Values


Reference values apply to all ages.

Clinical Reference

1. Mieli-Vergani G, Vergani D, Czaja AJ, et al: Autoimmune hepatitis. Nat Rev Dis Primers. 2018 Apr 12;4:18017

2. Terziroli Beretta-Piccoli B, Mieli-Vergani G, Vergani D: Serology in autoimmune hepatitis: A clinical-practice approach. Eur J Intern Med. 2018 Feb;48:35-43

3. Hennes EM, Zeniya M, Czaja AJ, et al: Simplified criteria for the diagnosis of autoimmune hepatitis. Hepatology. 2008 Jul;48(1):169-176

Method Description

The patient's serum in 1:20 and 1:40 (initial screening) dilutions is added to fresh tissue from mouse stomach/kidney and incubated; fluorescein-conjugated antiglobulin is then added. The slides are read with a fluorescence microscope.(Package insert: Kallestad Mouse Stomach/Kidney. Bio-Rad Laboratories, Inc; 01/2018)

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
SMAS Smooth Muscle Ab Screen, S 26971-2


Result ID Test Result Name Result LOINC Value
609515 Smooth Muscle Ab Screen, S 26971-2

Day(s) Performed

Monday through Saturday

Report Available

2 to 4 days
Inova Laboratories Additional Information:

Replaces AABG (Actin Antibody IgG)