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Test Code T4 T4, Total

Additional Codes

Epic: LAB126

Specimen Required

Collection Container/Type

Preferred: Serum gel

Acceptable: Green top (Lithium heparin)
Submission Container/Tube: Plastic vial
Specimen Volume: 3.0 mL
Collection Instructions: Centrifuge and separate cells after clot formation and within 4 hours of collection.  Remove serum if testing will be delayed more than 24 hours.

Specimen Minimum Volume

0.3 mL

Specimen Stability

Room temperature: Unacceptable
Refrigerated (on gel): 24 hours
Refrigerated (off gel): 6 days
Frozen: >6 days

Reference Range

4.87 - 11.72 µg/dL

Rejection Due To

Samples stored on gel >24 hours, unlabeled, mislabeled, wrong tube type, hemolyzed, QNS, exceeds specimen stability requirements.

Report Available

Same day

Clinical Significance

Thyroxine (T4) is an iodine-containing hormone and is secreted by the thyroid gland.  T4 and its associate thyroid hormone T3 are responsible for regulating diverse biochemical processes throughout the body.  T4 is 99.9% bound to serum thyroxine binding globulin.  Less than 0.05% of the total circulating T4 is unbound and therefore biologically active. Elevated T4 values are seen in patient with overt hyperthyroidism, while T4 levels are generally depressed in patient with hypothyroidism.

Method Name

Chemiluminescent microparticle immunoassay (CMIA)


The ARCHITECT Total T4 assay is designed to have a mean potential interference from hemoglobin, bilirubin, triglycerides, and protein of <10% at : 
Hemoglobin ≤ 500 mg/dL
Bilirubin ≤20 mg/dL
Triglycerides ≤ 3000 mg/dL
Protein ≥ 4.5 and ≤ 12 g/dL 



Day(s) Performed