Test Code TESTO Testosterone, Total
Additional Codes
Epic: LAB124
Specimen Required
Collection Container/Tube: Serum gel
Submission Container/Tube: Plastic vial
Specimen Volume: 3.0 mL
Collection Instructions: Centrifuge and separate
cells after clot formation and within 4 hours of collection.
Remove serum if testing will be delayed more than 8 hours.
Specimen Minimum Volume
0.10 mL
Specimen Stability
Room Temperature: 8 hours
Refrigerated (on gel): 8 hours
Refrigerated (off gel): 7 days
Frozen: 7 days
Reference Range
Males (adult): 241 - 827 ng/dL
Females (adult): 14 - 76 ng/dL
Rejection Due To
Samples stored on gel >8 hours, unlabeled, mislabeled, wrong tube type, hemolyzed, QNS, exceeds specimen stability requirements.
Report Available
Same day
Clinical Significance
The Architect 2nd Generation Testosterone assay is a
chemiluminescent microparticle immunoassay for the quantitative
determination of testosterone in human serum and plasma.
Testosterone measurements are used in the diagnosis and
treatment of disorders involving the male sex hormones, including
primary and secondary hypogonadism, delayed or precocious puberty,
impotence in males and, in females, hirsutism and virilization due
to tumors, polycystic ovaries, and andrenogenital syndromes.
The 2nd Generation Testosterone assay releases testosterone from
binding proteins and measures total testosterone. Free
testosterone can be calculated from the total testosterone, sex
hormone-binding globulin (SHBG) and albumin concentrations.
The concentration of testosterone fluctuates over 24 hours.
The pulsatile release of LH in the night typically leads to a
peak of testosterone concentration in the morning. Time of
day, age, sex, puberty, pre- and post-menopause, and disease, all
have an influence on testosterone concentration and should be
considered in interpreting individual results.
Method Name
Chemiluminescent Microparticle Immunoassay (CMIA)
Limitations
If the Testosterone results are inconsistent with clinical
evidence, additional testing is suggested to confirm the
result.
Specimens from patients who have received preparations of mouse
monoclonal antibodies for diagnosis or therapy may contain human
anti-mouse antibodies (HAMA). Such specimens may show either
falsely elevated or depressed values when tested with assay kits
that employ mouse monoclonal antibodies. Heterophilic antibodies in
human serum can react with reagent immunoglobulins, interfering
with in vitro immunoassays. Patients routinely exposed to animals
or to animal serum products can be prone to this interference and
anomalous values may be observed. Additional information may be
required for diagnosis.
LOINC
2986-8
Day(s) Performed
Daily