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HelpTest Code TTIGS Tetanus Toxoid IgG Antibody, Serum
Reporting Name
Tetanus Toxoid IgG Ab, SUseful For
Assessment of an antibody response to the tetanus toxoid vaccine, which should be performed at least 3 weeks after immunization
An aid to diagnose immunodeficiency
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumSpecimen Required
Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Specimen Volume: 0.5 mL
Specimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 30 days | |
| Frozen | 30 days |
Reference Values
Vaccinated: Positive (≥0.01 IU/mL)
Unvaccinated: Negative (<0.01 IU/mL)
Day(s) and Time(s) Performed
Monday through Friday; 9 a.m.
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
86317
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TTIGS | Tetanus Toxoid IgG Ab, S | 53935-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| TETG | Tetanus IgG Ab | 33469-8 |
| DEXTG | Tetanus IgG Value | 53935-3 |
Clinical Reference
1. Boland Birch T, Bleck TP: Tetanus (Clostridium tetani). In Bennett JE, Dolin R, Blaser MJ, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 9th ed. Elsevier; 2020:2948-2953
2. Gergen PJ, McQuillan GM, Kiely M, et al: A population-based serologic survey of immunity to tetanus in the United States. N Engl J Med. 1995;332:761-766
3. Bjorkholm B, Wahl M, Granstrom M, Hagberg L: Immune status and booster effects of low doses of tetanus toxoid in Swedish medical personnel. Scand J Infect Dis. 1994;26:471-475
4. Ramsay ME, Corbel MJ, Redhead K, et al: Persistence of antibody after accelerated immunization with diptheria/tetanus/pertussis vaccine. Br Med J. 1991;302:1489-1491
5. Rubin RL, Tang FL, Chan EK, et al: IgG subclasses of autoantibodies in systemic lupus erythematosus. Sjogren's syndrome, and drug-induced autoimmunitiy. J Immunol. 1986;137:2522-2527
6. Simonsen O, Bentzon MW, Heron I: ELISA for the routine determination of antitoxic immunity to tetanus. J Biol Stand. 1986;14:231-239
Method Description
The EuroImmun Anti-Tetanus Toxoid ELISA provides a quantitative in-vitro assay for detection of human IgG-class antibodies to Tetanus toxoid. The test kit contains reagent wells coated with tetanus toxoid. In the first reaction step, diluted patient samples are incubated in the wells. In the case of positive samples, specific IgG antibodies will bind to the antigens. To detect the bound antibodies, a second incubation is carried out using an enzyme-labelled anti-human IgG (enzyme conjugate) catalyzing a color reaction.(Package insert: Anti-Tetanus Toxoid ELISA (IgG) Test instruction. EuroImmun US; 08/18/2017)
Analytic Time
Same day/1 dayReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
Method Name
Enzyme Immunoassay (EIA)
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.