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Test Code VBTHI Thiamine (Vitamin B1), Whole Blood

Additional Codes

Mayo: TDP

Reporting Name

Thiamin (Vitamin B1), WB

Useful For

Assessment of thiamine deficiency


Measuring thiamine levels in patients with behavioral changes, eye signs, gait disturbances, delirium, and encephalopathy; or in patients with questionable nutritional status, especially those who appear at risk and who also are being given insulin for hyperglycemia

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Whole Blood EDTA

Shipping Instructions

Ship specimen in amber vial to protect from light.

Specimen Required

Patient Preparation: Fasting overnight (12-14 hours). Infants-draw prior to next feeding. Water can be taken as needed.

Supplies: Amber Frosted Tube, 5 mL (T192)

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Amber vial

Specimen Volume: 4 mL

Collection Instructions:

1. Invert 8 to 10 times to mix blood.

2. Transfer whole blood into amber vial or tube and freeze within 24 hours of collection.

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Frozen 28 days LIGHT PROTECTED

Reference Values

70-180 nmol/L

Day(s) and Time(s) Performed

Monday through Friday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
TDP Thiamin (Vitamin B1), WB 32554-8


Result ID Test Result Name Result LOINC Value
85753 Thiamin (Vitamin B1), WB 32554-8

Clinical Reference

1. Naidoo DP, Bramdev A, Cooper K: Wernicke's encephalopathy and alcohol-related disease. Postgrad Med J 1991;67;978-981

2. Herve C, Beyne P, Letteron PH, Delacoux E: Comparison of erythrocyte transketolase activity with thiamin and thiamin phosphate ester levels in chronic alcoholic patients. Clin Chim Acta 1995;234:91-100

3. Majumdar SK, Shaw GK, O'Gorman P, et al: Blood vitamin status (B1, B2, B6, folic acid, and B12) in patients with alcohol liver disease. Int J Vitam Nutr Res 1982;52:266-271

4. Ball GFM: Vitamins: Their role in the human body. Oxford, Blackwell Publishing, 2004, pp 273-288

5. Brin M: Erythrocyte as a biopsy tissue for functional evaluation of thiamin adequacy. JAMA 1964;187:762-766

6. Roberts NB. Taylor A. Sodi R: Chapter 37 Vitamins and Trace Elements. In Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. Edited by N Rifai, AR Horvath, CT Wittwer. Sixth edition. St. Louis, MO. Elsevier, 2018. pp 639-718

Method Description

Samples are extracted with analyte detection by liquid chromatography-tandem mass spectrometry.(Unpublished Mayo method)

Analytic Time

2 days (not reported Saturday or Sunday)

Reject Due To

Gross lipemia Reject
Other Glass vial Clotted specimen

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)


If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.

Secondary ID