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Test Code VITB1 Vitamin B1, Plasma/Serum

Specimen Required

2 mL frozen plasma

Specimen Minimum Volume

1.0 mL

Specimen Stability

Room temperature: 24 hours Refrigerated: 48 hours Frozen: 30 days

Rejection Due To

Hemolysis – Lipemia – Received room temperature – Received refrigerated – Not protected from light – Specimens collected in gel barrier tube – Specimens prepared from blood left at room temperature 4 hours or more – Serum Separator Tube (SST)

Day(s) and Time(s) Performed

Setup:  Tues – Sat a.m.

Method Name

Liquid Chromatography / Tandem Mass Spectrometry (LC/MS/MS)

Collection Container / Tube

Preferred: EDTA (lavendar-top) protect from light
Other Acceptable Container: Red Top Tube (no gel) protect from light.

Transport / Storage Conditions

Frozen

Special Instructions

Plasma(preferred):  Collect blood in an EDTA (lavendar-top) tube. Remove plasma within 4 hours of collection and transfer to a light protected pour off tube.  If separation of cells can't be perfromed immediately after collection, keep the whole  blood refrigerated and protected from light. The separation of cells must be completed within 4 hours of collection.  Separate cells by centrifugation at 2-8 C for 8 – 10 minutes.  Transfer plasma to dark brown polypropylene or polyethylene transport butres to protect from light and freeze tube immediately.  Alternately, neutral colored polypropylene or polyethylene tubes can be used if wrapped in aluminum foil. Ship frozen tubes at -10 to -30 C.

Serum (acceptable): Collect blood in plain red-top evaucated tube. Allow to clot at 10-25 C for 20-30 minutes. Centrifuge at 2-8 C for 8-10 minutes. Transfer serum to dark brown polypropylene or polyethylene transport tube to protect from light and freeze tube immediately.  Alternately, neutral colored polypropylene or polyethylene tubes can be used if wrapped in aluminum foil.  Ship the frozen tubes at -10 to -30 C.

Note:  Samples not protected from light or shipped at room or refrigerated temperature are unacceptable.

Reference Values

Adult:  8-30 nmol/L

Analytic Time

Report available:  3 days

Message

Plasma(preferred):  Collect blood in an EDTA (lavendar-top) tube. Remove plasma within 4 hours of collection and transfer to a light protected pour off tube.  If separation of cells can't be perfromed immediately after collection, keep the whole  blood refrigerated and protected from light. The separation of cells must be completed within 4 hours of collection.  Separate cells by centrifugation at 2-8 C for 8 – 10 minutes.  Transfer plasma to dark brown polypropylene or polyethylene transport butres to protect from light and freeze tube immediately.  Alternately, neutral colored polypropylene or polyethylene tubes can be used if wrapped in aluminum foil. Ship frozen tubes at -10 to -30 C.

Serum (acceptable): Collect blood in plain red-top evaucated tube. Allow to clot at 10-25 C for 20-30 minutes. Centrifuge at 2-8 C for 8-10 minutes. Transfer serum to dark brown polypropylene or polyethylene transport tube to protect from light and freeze tube immediately.  Alternately, neutral colored polypropylene or polyethylene tubes can be used if wrapped in aluminum foil.  Ship the frozen tubes at -10 to -30 C.

Note:  Samples not protected from light or shipped at room or refrigerated temperature are unacceptable.