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Test Code VITB3 Vitamin B3 and Metabolites, Plasma

Additional Codes

Epic: LAB1109

Mayo: VITB3

Specimen Required

Patient Preparation: Fasting 4 to 8 hours preferred

Supplies: Sarstedt 5 mL Aliquot Tube (T914)

Collection Container/Tube:

Preferred: Lavender top (K2 EDTA)

Acceptable: K3 EDTA

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions: Centrifuge and aliquot plasma into a plastic vial within 2 hours of collection.

Secondary ID


Useful For

Assisting in the diagnosis of suspected vitamin B3 deficiency or toxicity


May be useful in determining response to therapy

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Vitamin B3 and Metabolites, P

Specimen Type

Plasma EDTA

Specimen Minimum Volume

0.75 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma EDTA Refrigerated (preferred) 28 days
  Frozen  28 days
  Ambient  21 days

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Gross icterus OK

Reference Values

Nicotinic Acid (Niacin) Cutoff: <5.0 ng/mL

Nicotinamide: 5.0-48.0 ng/mL

Nicotinuric Acid Cutoff: <5.0 ng/mL

Clinical Reference

1. Delgado-Sanchez L, Godkar D, Niranjan S: Pellagra: rekindling of an old flame. Am J Ther. 2008 Mar-Apr;15(2):173-175. doi: 110.1097/MJT.1090b1013e31815ae31309

2. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA): A Scientific opinion on dietary reference values for niacin. EFSA Journal. 2014;12(7):3759. doi: 10.2903/j.efsa.2014.3759

3. Expert Group on Vitamins and Minerals: Safe Upper Levels for Vitamins and Minerals. Food Standard Agency; 2003:1-361. Accessed January 18, 2022. Available at

4. Fu CS, Swendseid ME, Jacob RA, McKee RW: Biochemical markers for assessment of niacin status in young men: Levels of erythrocyte niacin coenzymes and plasma tryptophan. J Nutr. 1989 Dec;119(12):1949-1955

5. Powers HJ: Current knowledge concerning optimum nutritional status of riboflavin, niacin and pyridoxine. Proc Nutr Soc. 1999 May;58(2):435-440

6. Shah GM, Shah RG, Veillette H, et al: Biochemical assessment of niacin deficiency among carcinoid cancer patients. Am J Gastroenterol. 2005 Oct;100(10): 2307-2314. doi: 2310.1111/j.1572-0241.2005.00268.x

7. Sun WP, Zhai MZ, Li D, et al: Comparison of the effects of nicotinic acid and nicotinamide degradation on plasma betaine and choline levels. Clin Nutr. 2017 Aug;36(4):1136-1142

Method Description

Vitamin B3 components (nicotinic acid/niacin, nicotinamide, nicotinuric acid) are extracted from plasma specimens with internal standard and then analyzed by liquid chromatography-tandem mass spectrometry.(Unpublished Mayo method)

Day(s) Performed

Monday, Tuesday, Thursday, Friday

Report Available

2 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
VITB3 Vitamin B3 and Metabolites, P In Process


Result ID Test Result Name Result LOINC Value
609493 Nicotinic Acid (Niacin) 18244-4
609494 Nicotinamide 56961-6
609495 Nicotinuric Acid 72306-4